Cinnamic acid and lactobionic acid based nanoformulations as a potential antiamoebic therapeutics

Noor Akbar, Muhammad Kawish, Tooba Jabri, Naveed Ahmed Khan, Muhammad Raza Shah, Ruqaiyyah Siddiqui

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)

Abstract

Acanthamoeba castellanii causes granulomatous amoebic encephalitis, an uncommon but severe brain infection and sight-threatening Acanthamoeba keratitis. Most of the currently used anti-amoebic treatments are not always effective, due to persistence of the cyst stage, and recurrence can occur. Here in this study we synthesize cinnamic acid and lactobionic acid-based magnetic nanoparticles (MNPs) using co-precipitation technique. These nanoformulations were characterized by Fourier transform infrared spectroscopy and Atomic form microscopy. The drugs alone (Hesperidin, Curcumin and Amphotericin B), magnetic NPs alone, and drug-loaded nano-formulations were evaluated at a concentration of 100 μg/mL for antiamoebic activity against a clinical isolate of A. castellanii. Amoebicidal assays revealed that drugs and conjugation of drugs and NPs further enhanced amoebicidal effects of drug-loaded nanoformulations. Drugs and drug-loaded nanoformulations inhibited both encystation and excystation of amoebae. In addition, drugs and drug-loaded nanoformulations inhibited parasite binding capability to the host cells. Neither drugs nor drug-loaded nanoformulations showed cytotoxic effects against host cells and considerably reduced parasite-mediated host cell death. Overall, these findings imply that conjugation of medically approved drugs with MNPs produce potent anti-Acanthamoebic effects, which could eventually lead to the development of therapeutic medications.

Original languageEnglish
Article number108474
JournalExperimental Parasitology
Volume246
Early online date25 Jan 2023
DOIs
Publication statusPublished - Mar 2023

Keywords

  • Humans
  • Metal Nanoparticles/chemistry
  • Amebiasis/parasitology
  • Amebicides/chemistry
  • Acanthamoeba castellanii

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