Chitinases from Vibrio: Activity screening and purification of chiA from Vibrio carchariae

W. Suginta, P. A W Robertson, B. Austin, S. C. Fry, L. A. Fothergill-Gilmore

    Research output: Contribution to journalArticle

    45 Citations (Scopus)

    Abstract

    Fourteen species of Vibrio were screened for chitin-induced chitinase activity in culture medium. V. carchariae, V. alginolyticus 283 and V. campbellii showed high levels of activity. Screening on agar plates containing swollen chitin showed high levels of chitinase activity by the same three species, and also by V. fischeri and V. alginolyticus 284. An affinity purification procedure was developed for the chitinase from V. carchariae. The purified chitinase was active as a monomer with M(r) 63 000- 66 000, and displayed activity toward polymeric chitin from acetylated chitosan or from crab shells. N-terminal sequence analysis and immunological cross-reactivity confirmed that the enzyme belongs to the group A/chiA family of bacterial chitinases.

    Original languageEnglish
    Pages (from-to)76-84
    Number of pages9
    JournalJournal of Applied Microbiology
    Volume89
    Issue number1
    DOIs
    Publication statusPublished - 2000

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