Characterization of the promoter of PRS1 in Saccharomyces cerevisiae identifies three regions potentially involved in control of expression

Yolanda Hernando, Andrew T Carter, Stefan Sickinger, Michael Schweizer

    Research output: Contribution to journalArticlepeer-review

    Abstract

    The transcription initiation site of the Saccharomyces cerevisiae PRS1 gene was mapped at -179 bp. Measurement of ß-galactosidase activity of the successively deleted PRS1 promoter linked to lacZ and integrated at the ura3 locus defined three DNA regions involved in the control of PRS1 expression. Gel shift analysis confirmed the data.

    Original languageEnglish
    Pages (from-to)795-799
    Number of pages5
    JournalJournal of Bacteriology
    Volume183
    Issue number2
    DOIs
    Publication statusPublished - Jan 2001

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