Characterization of Bacillus thuringiensis and related bacteria by ribosomal RNA gene restriction fragment length polymorphisms

F. G. Priest, D. A. Kaji, Y. B. Rosato, V. P. Canhos

    Research output: Contribution to journalArticle

    Abstract

    Ribosomal RNA gene restriction patterns have been determined for 43 strains of Bacillus thuringiensis representing 10 serovars and eight reference strains of B. anthracis, B. cereus and B. mycoides. Strains within a B. thuringiensis serovar produced highly related or identical ribotype patterns: in particular, 12 strains of serovar israelensis, five strains of serovar kurstaki, two strains of serovar galleriae and three strains of serovar aizawa produced ribotype patterns consistent with serotype designations. Moreover, variety tenebrionis (serotype 8a8b), a coleopteran pathogen, could be distinguished from the more common lepidopteran pathogens of this serotype (serovar morrisoni) by ribotyping. The correlation of ribotype patterns with serotype suggests a clonal population structure for B. thuringiensis.

    Original languageEnglish
    Pages (from-to)1015-1022
    Number of pages8
    JournalMicrobiology
    Volume140
    Issue number5
    Publication statusPublished - 1994

    Keywords

    • 16S rRNA genes
    • Bacillus thuringiensis
    • Ribotyping

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