Characterization of a maltose transport system in Clostridium acetobutylicum ATCC 824

M. Tangney, G. T. Winters, W. J. Mitchell

Research output: Contribution to journalArticle

Abstract

The utilization of maltose by Clostridium acetobutylicum ATCC 824 was investigated. Glucose was used preferentially to maltose, when both substrates were present in the medium. Maltose phosphoenolpyruvate (PEP)-dependent phosphotransferase system (PTS) activity was detected in extracts prepared from cultures grown on maltose, but not glucose or sucrose, as the sole carbon source. Extract fractionation and PTS reconstitution experiments revealed that the specificity for maltose is contained entirely within the membrane in this organism. A putative gene system for the maltose PTS was identified (from the C. acetobutylicum ATCC 824 genome sequence), encoding an enzyme IIMal and a maltose 6-phosphate hydrolase.

Original languageEnglish
Pages (from-to)298-306
Number of pages9
JournalJournal of Industrial Microbiology and Biotechnology
Volume27
Issue number5
DOIs
Publication statusPublished - 2001

Fingerprint

Clostridium acetobutylicum
Maltose
maltose-6'-phosphate glucosidase
Phosphotransferases
Glucose
Sucrose
Carbon
Genome
Membranes
Enzymes
Genes

Keywords

  • MalPH
  • Maltose 6-phosphate hydrolase
  • Phosphotransferase system

Cite this

Tangney, M. ; Winters, G. T. ; Mitchell, W. J. / Characterization of a maltose transport system in Clostridium acetobutylicum ATCC 824. In: Journal of Industrial Microbiology and Biotechnology. 2001 ; Vol. 27, No. 5. pp. 298-306.
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Characterization of a maltose transport system in Clostridium acetobutylicum ATCC 824. / Tangney, M.; Winters, G. T.; Mitchell, W. J.

In: Journal of Industrial Microbiology and Biotechnology, Vol. 27, No. 5, 2001, p. 298-306.

Research output: Contribution to journalArticle

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