Characterization and biosynthesis of non-degradable polymers in plant cuticles

J.F. Villena, E. Dominguez, D. Stewart, A. Heredia

    Research output: Contribution to journalArticle

    Abstract

    The structure and monomeric composition of the highly aliphatic and non-saponifiable fraction of cutans isolated from the leaf cuticles of Agave americana L. and Clivia miniata Reg. have been elucidated. Spectroscopic Fourier transform infrared and 13C-nuclear magnetic resonance, calorimetric and X-ray diffraction studies, together with biopolymer analysis after exhaustive ozonolysis, showed that the cutan fraction consists of an amorphous three-dimensional network linked by ether bonds containing double bonds and free carboxylic acid functions. Data obtained from fatty acid sorption indicated that the new biopolymer investigated here has a highly hydrophobic character constituting an additional barrier biopolymer in those cuticles where it is present. Labelled [14C]linoleic acid was preferentially incorporated into the non-ester part of C. miniata leaf disks in comparison with the cutin fraction of the cuticular membrane. This indicates that the cis-pentadiene system of polyunsaturated fatty acids is involved in the formation of intramolecular linkages, mainly ether bonds, of the aliphatic biopolymer.
    ©Springer-Verlag
    Original languageEnglish
    Pages (from-to)181-187
    Number of pages7
    JournalPlanta
    Volume208
    Issue number2
    DOIs
    Publication statusPublished - 1999

    Keywords

    • agave
    • aliphatic biopolymer
    • clivia
    • cutan
    • cuticle

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