Characteristics of maltose transporter activity in an ale and lager strain of the yeast Saccharomyces cerevisiae

R. M. Crumplen, J. C. Slaughter, G. G. Stewart

    Research output: Contribution to journalArticle

    23 Citations (Scopus)

    Abstract

    Kinetic studies on maltose uptake by ale and lager yeasts showed lower K(m) and higher V(max) values than for laboratory yeasts. Eadie-Hofstee plots were biphasic and inhibition by sulphydryl reagents was consistent with low affinity uptake by a specific transporter rather than by non-specific adsorption. High affinity maltose transport by both strains was inhibited by galactose, CCCP, 2,4-DNP and NEM. In contrast, glucose and pCMBS inhibited high affinity transport by the ale yeast but not by the lager yeast. In the case of pCMBS the difference could result from a single amino acid change in the maltose transporter.

    Original languageEnglish
    Pages (from-to)448-452
    Number of pages5
    JournalLetters in Applied Microbiology
    Volume23
    Issue number6
    DOIs
    Publication statusPublished - 1996

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