Abstract
This chapter focuses on methodologies suitable for mammalian cell separation as opposed to purification of other biological entities like particles, proteins and non-mammalian cells. It describes reported separation methods that may lend themselves to large-scale cell separation. Resolution requirements for the separation platform will be largely dictated by the nature of the starting population. Open versus closed systems for cell processing refers to the extent to which cells are exposed to the ambient environment, with a 'closed' environment limited by the boundaries of the vessel or device the cells are contained in. Cell-based products create new issues for bio-production and processing, requiring a re-think of current manufacturing strategy. The health economics of cell production and the therapeutic efficacy of the therapy on which it is based must be competitive with existing non-cellular treatments or management of a disease and its symptoms.
Original language | English |
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Title of host publication | Bioprocessing for Cell Based Therapies |
Editors | Che J. Connon |
Publisher | Wiley-Blackwell |
Pages | 127-165 |
Number of pages | 39 |
ISBN (Electronic) | 9781118743362 |
ISBN (Print) | 9781118743416 |
DOIs | |
Publication status | Published - 16 Dec 2016 |
Keywords
- Biological entities
- Closed environment
- Health economics
- Mammalian cell separation
- Non-cellular treatments
- Resolution requirements
ASJC Scopus subject areas
- General Biochemistry,Genetics and Molecular Biology