Cell death of Acanthamoeba castellanii following exposure to antimicrobial agents commonly included in contact lens disinfecting solutions

Louise Thomas, Naveed Ahmed Khan*, Ruqaiyyah Siddiqui, Bader S. Alawfi, David Lloyd*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

Several antimicrobial agents are commonly included in contact lens disinfectant solutions including chlorhexidine diacetate (CHX), polyhexamethylene biguanide (PHMB) or myristamidopropyl dimethylamine (MAPD); however, their mode of action, i.e. necrosis versus apoptosis is incompletely understood. Here, we determined whether a mechanism of cell death resembling that of apoptosis was present in Acanthamoeba castellanii of the T4 genotype (NEFF) following exposure to the aforementioned antimicrobials using the anticoagulant annexin V that undergoes rapid high affinity binding to phosphatidylserine in the presence of calcium, making it a sensitive probe for phosphatidylserine exposure. The results revealed that under the conditions employed in this study, an apoptotic pathway of cell death in this organism at the tested conditions does not occur. Our findings suggest that necrosis is the likely mode of action; however, future mechanistic studies should be accomplished in additional experimental conditions to further comprehend the molecular mechanisms of cell death in Acanthamoeba.

Original languageEnglish
Article number16
JournalParasitology Research
Volume123
Early online date7 Dec 2023
DOIs
Publication statusPublished - 2024

Keywords

  • Acanthamoeba castellanii
  • Amoebicidal
  • Contact lens disinfectants
  • Keratitis
  • Trophicidal

ASJC Scopus subject areas

  • Parasitology
  • veterinary(all)
  • Insect Science
  • Infectious Diseases

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