Cadmium-inducible expression of the yeast GSH1 gene requires a functional sulfur-amino acid regulatory network

Ulla Helene Dormer, John Westwater, Niall F McLaren, Nicholas A Kent, Jane Mellor, Derek James Jamieson

    Research output: Contribution to journalArticle

    Abstract

    Glutathione (?-L-glutamyl-L-cysteinylglycine) is an important antioxidant molecule, helping to buffer the cell against free radicals and toxic electrophiles. Expression of the yeast GSH1 gene, encoding the first enzyme involved in glutathione biosynthesis, ?-glutamylcysteine synthetase, is regulated by oxidants and the heavy metal cadmium at the level of transcription. We present evidence that the transcription factors involved in controlling the network of sulfur amino acid metabolism genes are also responsible for regulating GSH1 expression in response to cadmium. In particular the transcription factors Met-4, Met-31, and Met-32 are essential for cadmium-mediated regulation of gene expression, whereas the DNA-binding protein Cbf1 appears to play a negative role in controlling GSH1 expression.

    Original languageEnglish
    Pages (from-to)32611-32616
    Number of pages6
    JournalJournal of Biological Chemistry
    Volume275
    Issue number42
    DOIs
    Publication statusPublished - 20 Oct 2000

    Fingerprint

    sulfur amino acids
    cadmium
    yeasts
    glutathione
    transcription factors
    DNA-binding proteins
    genes
    amino acid metabolism
    gene expression regulation
    ligases
    oxidants
    heavy metals
    buffers
    transcription (genetics)
    biosynthesis
    antioxidants
    enzymes
    cells

    Cite this

    Dormer, Ulla Helene ; Westwater, John ; McLaren, Niall F ; Kent, Nicholas A ; Mellor, Jane ; Jamieson, Derek James. / Cadmium-inducible expression of the yeast GSH1 gene requires a functional sulfur-amino acid regulatory network. In: Journal of Biological Chemistry. 2000 ; Vol. 275, No. 42. pp. 32611-32616.
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    Cadmium-inducible expression of the yeast GSH1 gene requires a functional sulfur-amino acid regulatory network. / Dormer, Ulla Helene; Westwater, John; McLaren, Niall F; Kent, Nicholas A; Mellor, Jane; Jamieson, Derek James.

    In: Journal of Biological Chemistry, Vol. 275, No. 42, 20.10.2000, p. 32611-32616.

    Research output: Contribution to journalArticle

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    T1 - Cadmium-inducible expression of the yeast GSH1 gene requires a functional sulfur-amino acid regulatory network

    AU - Dormer, Ulla Helene

    AU - Westwater, John

    AU - McLaren, Niall F

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    AU - Mellor, Jane

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