Abstract
Glutathione (?-L-glutamyl-L-cysteinylglycine) is an important antioxidant molecule, helping to buffer the cell against free radicals and toxic electrophiles. Expression of the yeast GSH1 gene, encoding the first enzyme involved in glutathione biosynthesis, ?-glutamylcysteine synthetase, is regulated by oxidants and the heavy metal cadmium at the level of transcription. We present evidence that the transcription factors involved in controlling the network of sulfur amino acid metabolism genes are also responsible for regulating GSH1 expression in response to cadmium. In particular the transcription factors Met-4, Met-31, and Met-32 are essential for cadmium-mediated regulation of gene expression, whereas the DNA-binding protein Cbf1 appears to play a negative role in controlling GSH1 expression.
| Original language | English |
|---|---|
| Pages (from-to) | 32611-32616 |
| Number of pages | 6 |
| Journal | Journal of Biological Chemistry |
| Volume | 275 |
| Issue number | 42 |
| DOIs | |
| Publication status | Published - 20 Oct 2000 |
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Cadmium-inducible expression of the yeast GSH1 gene requires a functional sulfur-amino acid regulatory network. / Dormer, Ulla Helene; Westwater, John; McLaren, Niall F; Kent, Nicholas A; Mellor, Jane; Jamieson, Derek James.
In: Journal of Biological Chemistry, Vol. 275, No. 42, 20.10.2000, p. 32611-32616.Research output: Contribution to journal › Article
TY - JOUR
T1 - Cadmium-inducible expression of the yeast GSH1 gene requires a functional sulfur-amino acid regulatory network
AU - Dormer, Ulla Helene
AU - Westwater, John
AU - McLaren, Niall F
AU - Kent, Nicholas A
AU - Mellor, Jane
AU - Jamieson, Derek James
PY - 2000/10/20
Y1 - 2000/10/20
N2 - Glutathione (?-L-glutamyl-L-cysteinylglycine) is an important antioxidant molecule, helping to buffer the cell against free radicals and toxic electrophiles. Expression of the yeast GSH1 gene, encoding the first enzyme involved in glutathione biosynthesis, ?-glutamylcysteine synthetase, is regulated by oxidants and the heavy metal cadmium at the level of transcription. We present evidence that the transcription factors involved in controlling the network of sulfur amino acid metabolism genes are also responsible for regulating GSH1 expression in response to cadmium. In particular the transcription factors Met-4, Met-31, and Met-32 are essential for cadmium-mediated regulation of gene expression, whereas the DNA-binding protein Cbf1 appears to play a negative role in controlling GSH1 expression.
AB - Glutathione (?-L-glutamyl-L-cysteinylglycine) is an important antioxidant molecule, helping to buffer the cell against free radicals and toxic electrophiles. Expression of the yeast GSH1 gene, encoding the first enzyme involved in glutathione biosynthesis, ?-glutamylcysteine synthetase, is regulated by oxidants and the heavy metal cadmium at the level of transcription. We present evidence that the transcription factors involved in controlling the network of sulfur amino acid metabolism genes are also responsible for regulating GSH1 expression in response to cadmium. In particular the transcription factors Met-4, Met-31, and Met-32 are essential for cadmium-mediated regulation of gene expression, whereas the DNA-binding protein Cbf1 appears to play a negative role in controlling GSH1 expression.
UR - http://www.scopus.com/inward/record.url?scp=0034693270&partnerID=8YFLogxK
U2 - 10.1074/jbc.M004167200
DO - 10.1074/jbc.M004167200
M3 - Article
VL - 275
SP - 32611
EP - 32616
JO - Journal of Biological Chemistry
JF - Journal of Biological Chemistry
SN - 0021-9258
IS - 42
ER -