Beta1-integrin and PTEN control the phosphorylation of protein kinase C

Davey B Parekh, Roy M T Katso, Nick R Leslie, C Peter Downes, Katarzyna J Procyk, Michael D Waterfield, Peter J Parker

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39 Citations (Scopus)

Abstract

Phosphorylation of protein kinase C (PKC) provides an amplitude control that operates in conjunction with allosteric effectors. Under many conditions, PKC isotypes appear to be highly phosphorylated; however, the cellular inputs that maintain these phosphorylations are not characterized. In the present work, it is shown that there is a differential phosphorylation of PKCdelta in adherent versus suspension cultures of transfected HEK-293 cells. It is established that integrin activation is sufficient to trigger PKCdelta phosphorylation and that this signals through phosphoinositide 3-kinase (PI3-kinase) to stimulate the phosphorylation of two sites, T505 and S662. The loss of signal input to PKCdelta in suspension culture is dependent on the tumour suppressor gene PTEN, which encodes a bi-functional phosphotyrosine/phosphoinositide 3-phosphate phosphatase. In the PTEN(-/-) UM-UC-3 bladder carcinoma cell line grown in suspension, transfected PKCdelta no longer accumulates in a dephospho-form on serum removal. By contrast, in a UM-UC-3-derivative cell line stably expressing PTEN, PKCdelta does become dephosphorylated under these conditions. Employing the PTEN Gly(129)-->Glu mutant, which is selectively defective in lipid phosphatase activity, it was established that it is the lipid phosphatase activity that controls PKCdelta phosphorylation. The evidence indicates that PKCdelta phosphorylation and its latent activity are maintained in serum-deprived adherent cultures through integrin-matrix interactions. This control acts through a pathway involving a lipid product of PI3-kinase in a manner that can be suppressed by PTEN.
Original languageEnglish
Pages (from-to)425-433
Number of pages9
JournalBiochemical Journal
Volume352
Issue number2
Publication statusPublished - 1 Dec 2000

Keywords

  • Antigens, CD29
  • Cell Line
  • Enzyme Activation
  • Humans
  • Isoenzymes
  • PTEN Phosphohydrolase
  • Phosphatidylinositol 3-Kinases
  • Phosphoric Monoester Hydrolases
  • Phosphorylation
  • Protein Kinase C
  • Protein Kinase C-delta
  • Tumor Suppressor Proteins

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    Parekh, D. B., Katso, R. M. T., Leslie, N. R., Downes, C. P., Procyk, K. J., Waterfield, M. D., & Parker, P. J. (2000). Beta1-integrin and PTEN control the phosphorylation of protein kinase C. Biochemical Journal, 352(2), 425-433.