TY - JOUR
T1 - Association of Hg2+ with aqueous (C60)n aggregates facilitates increased bioavailability of Hg2+ in zebrafish (Danio rerio)
AU - Henry, Ted
AU - Wileman, Stephen J
AU - Boran, Halis
AU - Sutton, Paul
PY - 2013/9/3
Y1 - 2013/9/3
N2 - Manufactured nanoparticles (NPs) can associate with toxicants in the aqueous phase and these associations can influence the environmental fate, transport, and bioavailability of these toxicants in organisms. Dissolved metals (e.g., Hg2+) can be toxic in aquatic organisms, and, if metals associate with NPs in the aqueous phase, changes in bioavailability and toxicology may result. Here we demonstrated that Hg2+ (25 µg/L) can associate with aqueous (C60)n (termed nC60) and increase aggregate size and settlement of nC60 aggregates out of the water column over 24 h. The concentration of C60 was directly related to concentration of Hg for nC60 aggregates that settled to the bottom of the container. Bioavailability of Hg2+ in larval zebrafish Danio rerio, evaluated by assessment of metallothionein gene (mt2) expression, was reduced in the water column when nC60was present. However, zebrafish residing at the container bottom and exposed to nC60 aggregates with associated Hg2+ had elevated expression of mt2 when compared to fish exposed to 25 µg/L Hg2+ preparations without nC60, which indicated nC60 led to a localized increase in Hg2+ bioavailability. Results indicate that aqueous nC60 can sorb Hg2+, transport Hg2+ to substrate surface, and increase concentrations of bioavailable Hg2+ in organisms located where settled nC60 aggregates accumulate.
AB - Manufactured nanoparticles (NPs) can associate with toxicants in the aqueous phase and these associations can influence the environmental fate, transport, and bioavailability of these toxicants in organisms. Dissolved metals (e.g., Hg2+) can be toxic in aquatic organisms, and, if metals associate with NPs in the aqueous phase, changes in bioavailability and toxicology may result. Here we demonstrated that Hg2+ (25 µg/L) can associate with aqueous (C60)n (termed nC60) and increase aggregate size and settlement of nC60 aggregates out of the water column over 24 h. The concentration of C60 was directly related to concentration of Hg for nC60 aggregates that settled to the bottom of the container. Bioavailability of Hg2+ in larval zebrafish Danio rerio, evaluated by assessment of metallothionein gene (mt2) expression, was reduced in the water column when nC60was present. However, zebrafish residing at the container bottom and exposed to nC60 aggregates with associated Hg2+ had elevated expression of mt2 when compared to fish exposed to 25 µg/L Hg2+ preparations without nC60, which indicated nC60 led to a localized increase in Hg2+ bioavailability. Results indicate that aqueous nC60 can sorb Hg2+, transport Hg2+ to substrate surface, and increase concentrations of bioavailable Hg2+ in organisms located where settled nC60 aggregates accumulate.
U2 - 10.1021/es4015597
DO - 10.1021/es4015597
M3 - Article
C2 - 23941233
AN - SCOPUS:84883499057
SN - 0013-936X
VL - 47
SP - 9997
EP - 10004
JO - Environmental Science and Technology
JF - Environmental Science and Technology
IS - 17
ER -