Assessment of the use of compost stability as an indicator of alkane and aromatic hydrocarbon degrader abundance in green waste composting materials and finished composts for soil bioremediation application

Diana Guillen Ferrari; Jennifer Pratscher; Thomas J. Aspray

doi:10.1016/j.wasman.2019.06.030

Assessment of the use of compost stability as an indicator of alkane and aromatic hydrocarbon degrader abundance in green waste composting materials and finished composts for soil bioremediation application

Diana Guillen Ferrari, Jennifer Pratscher, Thomas J. Aspray^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

6 Citations (Scopus)

17 Downloads (Pure)

Abstract

Green waste composting materials and finished composts were collected from different commercial ex situ composting sites all treating source segregated green waste feedstocks. Stability of each material was determined using the standard ORG0020 dynamic respiration test. To assess whether stability could be used as an indicator for the potential suitability of green waste composting materials and finished composts as amendments for soil bioremediation, comparison was made with alkane and aromatic hydrocarbon degrader abundance determined using a quantitative PCR (qPCR) approach. Specifically, primers targeting alkB and, polyaromatic hydrocarbon ring-hydroxylating dioxygenases genes (PAH-RHD) of Gram positive (GP) and Gram negative (GN) populations were used for qPCR analysis. The results showed no direct correction between compost stability and gene abundance. Further, increase in alkB gene abundance was not linked to PAH-RHD gene abundance. The results support the use of qPCR as a tool for screening organic amendments on a site by site basis for soil bioremediation treatment.

Original language	English
Pages (from-to)	365-369
Number of pages	5
Journal	Waste Management
Volume	95
Early online date	24 Jun 2019
DOIs	https://doi.org/10.1016/j.wasman.2019.06.030
Publication status	Published - 15 Jul 2019

Keywords

Alkane
Bioremediation
Compost
Stability
Total petroleum hydrocarbons

ASJC Scopus subject areas

Waste Management and Disposal

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10.1016/j.wasman.2019.06.030

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title = "Assessment of the use of compost stability as an indicator of alkane and aromatic hydrocarbon degrader abundance in green waste composting materials and finished composts for soil bioremediation application",

abstract = "Green waste composting materials and finished composts were collected from different commercial ex situ composting sites all treating source segregated green waste feedstocks. Stability of each material was determined using the standard ORG0020 dynamic respiration test. To assess whether stability could be used as an indicator for the potential suitability of green waste composting materials and finished composts as amendments for soil bioremediation, comparison was made with alkane and aromatic hydrocarbon degrader abundance determined using a quantitative PCR (qPCR) approach. Specifically, primers targeting alkB and, polyaromatic hydrocarbon ring-hydroxylating dioxygenases genes (PAH-RHD) of Gram positive (GP) and Gram negative (GN) populations were used for qPCR analysis. The results showed no direct correction between compost stability and gene abundance. Further, increase in alkB gene abundance was not linked to PAH-RHD gene abundance. The results support the use of qPCR as a tool for screening organic amendments on a site by site basis for soil bioremediation treatment.",

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author = "{Guillen Ferrari}, Diana and Jennifer Pratscher and Aspray, {Thomas J.}",

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Assessment of the use of compost stability as an indicator of alkane and aromatic hydrocarbon degrader abundance in green waste composting materials and finished composts for soil bioremediation application. / Guillen Ferrari, Diana; Pratscher, Jennifer; Aspray, Thomas J.

In: Waste Management, Vol. 95, 15.07.2019, p. 365-369.

Research output: Contribution to journal › Article › peer-review

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AU - Pratscher, Jennifer

AU - Aspray, Thomas J.

PY - 2019/7/15

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N2 - Green waste composting materials and finished composts were collected from different commercial ex situ composting sites all treating source segregated green waste feedstocks. Stability of each material was determined using the standard ORG0020 dynamic respiration test. To assess whether stability could be used as an indicator for the potential suitability of green waste composting materials and finished composts as amendments for soil bioremediation, comparison was made with alkane and aromatic hydrocarbon degrader abundance determined using a quantitative PCR (qPCR) approach. Specifically, primers targeting alkB and, polyaromatic hydrocarbon ring-hydroxylating dioxygenases genes (PAH-RHD) of Gram positive (GP) and Gram negative (GN) populations were used for qPCR analysis. The results showed no direct correction between compost stability and gene abundance. Further, increase in alkB gene abundance was not linked to PAH-RHD gene abundance. The results support the use of qPCR as a tool for screening organic amendments on a site by site basis for soil bioremediation treatment.

AB - Green waste composting materials and finished composts were collected from different commercial ex situ composting sites all treating source segregated green waste feedstocks. Stability of each material was determined using the standard ORG0020 dynamic respiration test. To assess whether stability could be used as an indicator for the potential suitability of green waste composting materials and finished composts as amendments for soil bioremediation, comparison was made with alkane and aromatic hydrocarbon degrader abundance determined using a quantitative PCR (qPCR) approach. Specifically, primers targeting alkB and, polyaromatic hydrocarbon ring-hydroxylating dioxygenases genes (PAH-RHD) of Gram positive (GP) and Gram negative (GN) populations were used for qPCR analysis. The results showed no direct correction between compost stability and gene abundance. Further, increase in alkB gene abundance was not linked to PAH-RHD gene abundance. The results support the use of qPCR as a tool for screening organic amendments on a site by site basis for soil bioremediation treatment.

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KW - Stability

KW - Total petroleum hydrocarbons

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Assessment of the use of compost stability as an indicator of alkane and aromatic hydrocarbon degrader abundance in green waste composting materials and finished composts for soil bioremediation application

Abstract

Keywords

ASJC Scopus subject areas

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