Assessment of DNA damage in sperm after repeated non-invasive sampling in zebrafish Danio rerio

H. C. Reinardy; E. Skippins; T. B. Henry; A. N. Jha

doi:10.1111/jfb.12042

Assessment of DNA damage in sperm after repeated non-invasive sampling in zebrafish Danio rerio

H. C. Reinardy, E. Skippins, T. B. Henry^*, A. N. Jha

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

16 Citations (Scopus)

Abstract

Repeated non-invasive sampling of zebrafish Danio rerio sperm was conducted, sperm counts were obtained and a method for measurement of DNA damage in sperm was developed and validated (single-cell gel electrophoresis, comet, assay). DNA damage in sperm increased with concentration of hydrogen peroxide (H2O2, 0200 mu M), and in vitro exposure of sperm to 200 mu M H2O2 produced 88 center dot 7 +/- 3 center dot 9% tail DNA compared to unexposed controls [12 +/- 0 center dot 7% tail DNA (mean +/- s.e., n=3)]. Frequency of sperm sampling (sampled every 2, 4 or 7 days) did not affect DNA damage in sperm, but sperm counts decreased 57 and 22% for fish sampled every 2 or 4days, respectively.

Original language	English
Pages (from-to)	1074-1081
Number of pages	8
Journal	Journal of Fish Biology
Volume	82
Issue number	3
DOIs	https://doi.org/10.1111/jfb.12042
Publication status	Published - Mar 2013

Keywords

comet assay
COMET ASSAY
DNA strand breaks
EXPOSURE
hydrogen peroxide
SPERMATOZOA
repeated sampling
single-cell gel electrophoresis assay

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10.1111/jfb.12042

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title = "Assessment of DNA damage in sperm after repeated non-invasive sampling in zebrafish Danio rerio",

abstract = "Repeated non-invasive sampling of zebrafish Danio rerio sperm was conducted, sperm counts were obtained and a method for measurement of DNA damage in sperm was developed and validated (single-cell gel electrophoresis, comet, assay). DNA damage in sperm increased with concentration of hydrogen peroxide (H2O2, 0200 mu M), and in vitro exposure of sperm to 200 mu M H2O2 produced 88 center dot 7 +/- 3 center dot 9% tail DNA compared to unexposed controls [12 +/- 0 center dot 7% tail DNA (mean +/- s.e., n=3)]. Frequency of sperm sampling (sampled every 2, 4 or 7 days) did not affect DNA damage in sperm, but sperm counts decreased 57 and 22% for fish sampled every 2 or 4days, respectively.",

keywords = "comet assay, COMET ASSAY, DNA strand breaks, EXPOSURE, hydrogen peroxide, SPERMATOZOA, repeated sampling, single-cell gel electrophoresis assay",

author = "Reinardy, {H. C.} and E. Skippins and Henry, {T. B.} and Jha, {A. N.}",

year = "2013",

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doi = "10.1111/jfb.12042",

language = "English",

volume = "82",

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TY - JOUR

T1 - Assessment of DNA damage in sperm after repeated non-invasive sampling in zebrafish Danio rerio

AU - Reinardy, H. C.

AU - Skippins, E.

AU - Henry, T. B.

AU - Jha, A. N.

PY - 2013/3

Y1 - 2013/3

N2 - Repeated non-invasive sampling of zebrafish Danio rerio sperm was conducted, sperm counts were obtained and a method for measurement of DNA damage in sperm was developed and validated (single-cell gel electrophoresis, comet, assay). DNA damage in sperm increased with concentration of hydrogen peroxide (H2O2, 0200 mu M), and in vitro exposure of sperm to 200 mu M H2O2 produced 88 center dot 7 +/- 3 center dot 9% tail DNA compared to unexposed controls [12 +/- 0 center dot 7% tail DNA (mean +/- s.e., n=3)]. Frequency of sperm sampling (sampled every 2, 4 or 7 days) did not affect DNA damage in sperm, but sperm counts decreased 57 and 22% for fish sampled every 2 or 4days, respectively.

AB - Repeated non-invasive sampling of zebrafish Danio rerio sperm was conducted, sperm counts were obtained and a method for measurement of DNA damage in sperm was developed and validated (single-cell gel electrophoresis, comet, assay). DNA damage in sperm increased with concentration of hydrogen peroxide (H2O2, 0200 mu M), and in vitro exposure of sperm to 200 mu M H2O2 produced 88 center dot 7 +/- 3 center dot 9% tail DNA compared to unexposed controls [12 +/- 0 center dot 7% tail DNA (mean +/- s.e., n=3)]. Frequency of sperm sampling (sampled every 2, 4 or 7 days) did not affect DNA damage in sperm, but sperm counts decreased 57 and 22% for fish sampled every 2 or 4days, respectively.

KW - comet assay

KW - COMET ASSAY

KW - DNA strand breaks

KW - EXPOSURE

KW - hydrogen peroxide

KW - SPERMATOZOA

KW - repeated sampling

KW - single-cell gel electrophoresis assay

U2 - 10.1111/jfb.12042

DO - 10.1111/jfb.12042

M3 - Article

C2 - 23464563

SN - 0022-1112

VL - 82

SP - 1074

EP - 1081

JO - Journal of Fish Biology

JF - Journal of Fish Biology

IS - 3

ER -

Assessment of DNA damage in sperm after repeated non-invasive sampling in zebrafish Danio rerio

Abstract

Keywords

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