Assessment of DNA damage in sperm after repeated non-invasive sampling in zebrafish Danio rerio

H. C. Reinardy, E. Skippins, T. B. Henry, A. N. Jha

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    Abstract

    Repeated non-invasive sampling of zebrafish Danio rerio sperm was conducted, sperm counts were obtained and a method for measurement of DNA damage in sperm was developed and validated (single-cell gel electrophoresis, comet, assay). DNA damage in sperm increased with concentration of hydrogen peroxide (H2O2, 0200 mu M), and in vitro exposure of sperm to 200 mu M H2O2 produced 88 center dot 7 +/- 3 center dot 9% tail DNA compared to unexposed controls [12 +/- 0 center dot 7% tail DNA (mean +/- s.e., n=3)]. Frequency of sperm sampling (sampled every 2, 4 or 7 days) did not affect DNA damage in sperm, but sperm counts decreased 57 and 22% for fish sampled every 2 or 4days, respectively.

    Original languageEnglish
    Pages (from-to)1074-1081
    Number of pages8
    JournalJournal of Fish Biology
    Volume82
    Issue number3
    DOIs
    Publication statusPublished - Mar 2013

    Keywords

    • comet assay
    • COMET ASSAY
    • DNA strand breaks
    • EXPOSURE
    • hydrogen peroxide
    • SPERMATOZOA
    • repeated sampling
    • single-cell gel electrophoresis assay

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