TY - JOUR
T1 - Assessing the impact of nitrogen supplementation in oats across multiple growth locations and years with targeted phenotyping and high-resolution metabolite profiling approaches
AU - William Allwood, J.
AU - Martinez-Martin, Pilar
AU - Xu, Yun
AU - Cowan, Alexander
AU - Pont, Simon
AU - Griffiths, Irene
AU - Sungurtas, Julie
AU - Clarke, Sarah
AU - Goodacre, Royston
AU - Marshall, Athole
AU - Stewart, Derek
AU - Howarth, Catherine
N1 - Funding Information:
The authors would like to thank and acknowledge the support of this work by the BBSRC funded project, Developing enhanced breeding methodologies for oats for human health and nutrition (BBSRC grant No. BB/M000869/1). CH and PMM would also like to thank and acknowledge support of this work from AHDB projects. 2114002 and 21120003 and from BBSRC grant BBS/E/W/0012843B. JWA and DS acknowledge support from the Scottish Government Rural and Environmental Science and Analytical Services. JWA would like to thank Dr. Robert Hancock (James Hutton Institute) for his critical evaluation of the manuscript.
Funding Information:
The authors would like to thank and acknowledge the support of this work by the BBSRC funded project, Developing enhanced breeding methodologies for oats for human health and nutrition (BBSRC grant No. BB/M000869/1). CH and PMM would also like to thank and acknowledge support of this work from AHDB projects. 2114002 and 21120003 and from BBSRC grant BBS/E/W/0012843B. JWA and DS acknowledge support from the Scottish Government Rural and Environmental Science and Analytical Services. JWA would like to thank Dr. Robert Hancock (James Hutton Institute) for his critical evaluation of the manuscript. GC-MS and UHPLC-MS data have been deposited to the EMBL-EBI MetaboLights database (DOI: 10.1093/nar/gks1004. PubMed PMID: 23109552) with the identifier MTBLS1801. The complete dataset can be accessed here https://www.ebi.ac.uk/metabolights/MTBLS1801.
Publisher Copyright:
© 2021 The Authors
PY - 2021/9/1
Y1 - 2021/9/1
N2 - Oats (Avena sativa L.) are a healthy food, being high in dietary fibre (e.g. β-glucans), antioxidants, minerals, and vitamins. Understanding the effect of variety and crop management on nutritional quality is important. The response of four oat varieties to increased nitrogen levels was investigated across multiple locations and years with respect to yield, grain quality and metabolites (assessed via GC- and LC- MS). A novel high-resolution UHPLC-PDA-MS/MS method was developed, providing improved metabolite enrichment, resolution, and identification. The combined phenotyping approach revealed that, amino acid levels were increased by nitrogen supplementation, as were total protein and nitrogen containing lipid levels, whereas health-beneficial avenanthramides were decreased. Although nitrogen addition significantly increased grain yield and β-glucan content, supporting increasing the total nitrogen levels recommended within agricultural guidelines, oat varietal choice as well as negative impacts upon health beneficial secondary metabolites and the environmental burdens associated with nitrogen fertilisation, require further consideration.
AB - Oats (Avena sativa L.) are a healthy food, being high in dietary fibre (e.g. β-glucans), antioxidants, minerals, and vitamins. Understanding the effect of variety and crop management on nutritional quality is important. The response of four oat varieties to increased nitrogen levels was investigated across multiple locations and years with respect to yield, grain quality and metabolites (assessed via GC- and LC- MS). A novel high-resolution UHPLC-PDA-MS/MS method was developed, providing improved metabolite enrichment, resolution, and identification. The combined phenotyping approach revealed that, amino acid levels were increased by nitrogen supplementation, as were total protein and nitrogen containing lipid levels, whereas health-beneficial avenanthramides were decreased. Although nitrogen addition significantly increased grain yield and β-glucan content, supporting increasing the total nitrogen levels recommended within agricultural guidelines, oat varietal choice as well as negative impacts upon health beneficial secondary metabolites and the environmental burdens associated with nitrogen fertilisation, require further consideration.
KW - Avenanthramides
KW - Grain quality
KW - Lipids
KW - Metabolomics
KW - Nitrogen
KW - Oats (Avena sativa L.)
KW - Proteins and amino acids
KW - β-glucan
UR - http://www.scopus.com/inward/record.url?scp=85106069207&partnerID=8YFLogxK
U2 - 10.1016/j.foodchem.2021.129585
DO - 10.1016/j.foodchem.2021.129585
M3 - Article
C2 - 33799237
SN - 0308-8146
VL - 355
JO - Food Chemistry
JF - Food Chemistry
M1 - 129585
ER -