Assaying PTEN catalysis in vitro

Laura Spinelli; Nicholas R. Leslie

doi:10.1016/j.ymeth.2014.11.003

Assaying PTEN catalysis in vitro

Laura Spinelli, Nicholas R. Leslie^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

8 Citations (Scopus)

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Abstract

PTEN is a major tumour suppressor protein and a regulator of numerous diverse biological processes. It has an evolutionarily conserved role as a phosphoinositide lipid phosphatase, regulating the PI3K signalling pathway, but also has catalytic phosphatase activity against protein substrates, although the significance of this latter activity is less well understood. Unlike many tumour suppressors, even modest changes in PTEN activity can have strong effects on phenotypes, including tumour formation. Due to this recognised functional significance, several experimental platforms have been developed to assay the catalytic activity of PTEN against different substrates and are being applied to understand this cellular substrate diversity and the regulation of PTEN. Here we present and discuss methods to assay the phosphatase activity of PTEN in vitro. (C) 2014 Elsevier Inc. All rights reserved.

Original language	English
Pages (from-to)	51-57
Number of pages	7
Journal	Methods
Volume	77-78
DOIs	https://doi.org/10.1016/j.ymeth.2014.11.003
Publication status	Published - 1 May 2015

Keywords

Phosphatase
Tumour suppressor
Phosphoinositide
Vesicle
Enzyme assay
Phosphate
TUMOR-SUPPRESSOR PTEN
PHOSPHATASE-ACTIVITY
PHOSPHOINOSITIDE PHOSPHATASES
CELLS
GROWTH
INS(1,3,4,5,6)P-5
3-PHOSPHATASE
INACTIVATION
3-KINASES
MECHANISM

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title = "Assaying PTEN catalysis in vitro",

abstract = "PTEN is a major tumour suppressor protein and a regulator of numerous diverse biological processes. It has an evolutionarily conserved role as a phosphoinositide lipid phosphatase, regulating the PI3K signalling pathway, but also has catalytic phosphatase activity against protein substrates, although the significance of this latter activity is less well understood. Unlike many tumour suppressors, even modest changes in PTEN activity can have strong effects on phenotypes, including tumour formation. Due to this recognised functional significance, several experimental platforms have been developed to assay the catalytic activity of PTEN against different substrates and are being applied to understand this cellular substrate diversity and the regulation of PTEN. Here we present and discuss methods to assay the phosphatase activity of PTEN in vitro. (C) 2014 Elsevier Inc. All rights reserved.",

keywords = "Phosphatase, Tumour suppressor, Phosphoinositide, Vesicle, Enzyme assay, Phosphate, TUMOR-SUPPRESSOR PTEN, PHOSPHATASE-ACTIVITY, PHOSPHOINOSITIDE PHOSPHATASES, CELLS, GROWTH, INS(1,3,4,5,6)P-5, 3-PHOSPHATASE, INACTIVATION, 3-KINASES, MECHANISM",

author = "Laura Spinelli and Leslie, {Nicholas R.}",

note = "{"}This work was funded by the Medical Research Council (grant code G0801865) and in the University of Dundee by the pharmaceutical companies of the DSTT consortium (Astra Zeneca, Boehringer Ingelheim, GlaxoSmithKline, Merck KGaA and Pfizer). {"}",

year = "2015",

month = may,

day = "1",

doi = "10.1016/j.ymeth.2014.11.003",

language = "English",

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pages = "51--57",

journal = "Methods",

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TY - JOUR

T1 - Assaying PTEN catalysis in vitro

AU - Spinelli, Laura

AU - Leslie, Nicholas R.

N1 - "This work was funded by the Medical Research Council (grant code G0801865) and in the University of Dundee by the pharmaceutical companies of the DSTT consortium (Astra Zeneca, Boehringer Ingelheim, GlaxoSmithKline, Merck KGaA and Pfizer). "

PY - 2015/5/1

Y1 - 2015/5/1

N2 - PTEN is a major tumour suppressor protein and a regulator of numerous diverse biological processes. It has an evolutionarily conserved role as a phosphoinositide lipid phosphatase, regulating the PI3K signalling pathway, but also has catalytic phosphatase activity against protein substrates, although the significance of this latter activity is less well understood. Unlike many tumour suppressors, even modest changes in PTEN activity can have strong effects on phenotypes, including tumour formation. Due to this recognised functional significance, several experimental platforms have been developed to assay the catalytic activity of PTEN against different substrates and are being applied to understand this cellular substrate diversity and the regulation of PTEN. Here we present and discuss methods to assay the phosphatase activity of PTEN in vitro. (C) 2014 Elsevier Inc. All rights reserved.

AB - PTEN is a major tumour suppressor protein and a regulator of numerous diverse biological processes. It has an evolutionarily conserved role as a phosphoinositide lipid phosphatase, regulating the PI3K signalling pathway, but also has catalytic phosphatase activity against protein substrates, although the significance of this latter activity is less well understood. Unlike many tumour suppressors, even modest changes in PTEN activity can have strong effects on phenotypes, including tumour formation. Due to this recognised functional significance, several experimental platforms have been developed to assay the catalytic activity of PTEN against different substrates and are being applied to understand this cellular substrate diversity and the regulation of PTEN. Here we present and discuss methods to assay the phosphatase activity of PTEN in vitro. (C) 2014 Elsevier Inc. All rights reserved.

KW - Phosphatase

KW - Tumour suppressor

KW - Phosphoinositide

KW - Vesicle

KW - Enzyme assay

KW - Phosphate

KW - TUMOR-SUPPRESSOR PTEN

KW - PHOSPHATASE-ACTIVITY

KW - PHOSPHOINOSITIDE PHOSPHATASES

KW - CELLS

KW - GROWTH

KW - INS(1,3,4,5,6)P-5

KW - 3-PHOSPHATASE

KW - INACTIVATION

KW - 3-KINASES

KW - MECHANISM

U2 - 10.1016/j.ymeth.2014.11.003

DO - 10.1016/j.ymeth.2014.11.003

M3 - Article

C2 - 25461809

SN - 1046-2023

VL - 77-78

SP - 51

EP - 57

JO - Methods

JF - Methods

ER -

Assaying PTEN catalysis in vitro

Abstract

Keywords

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