Arabidopsis mitogen-activated protein kinase kinases MKK1 and MKK2 have overlapping functions in defense signaling mediated by MEKK1, MPK4, and MKS1

Jin Long Qiu, Lu Zhou, Byung Wook Yun, H. B. Nielsen, Berthe Katrine Fiil, Klaus Petersen, James Mackinlay, Gary J. Loake, John Mundy, Peter C. Morris

    Research output: Contribution to journalArticle

    197 Citations (Scopus)

    Abstract

    The Arabidopsis (Arabidopsis thaliana) MKK1 and MKK2 mitogen-activated protein kinase kinases have been implicated in biotic and abiotic stress responses as part of a signaling cascade including MEKK1 and MPK4. Here, the double loss-of-function mutant (mkk1/2) of MKK1 and MKK2 is shown to have marked phenotypes in development and disease resistance similar to those of the single mekk1 and mpk4 mutants. Because mkk1 or mkk2 single mutants appear wild type, basal levels of MPK4 activity are not impaired in them, and MKK1 and MKK2 are in part functionally redundant in unchallenged plants. These findings are confirmed and extended by biochemical and molecular analyses implicating the kinases in jasmonate- and salicylate-dependent defense responses, mediated in part via the MPK4 substrate MKS1. In addition, transcriptome analyses delineate overlapping and specific effects of the kinases on global gene expression patterns demonstrating both redundant and unique functions for MKK1 and MKK2. © 2008 American Society of Plant Biologists.

    Original languageEnglish
    Pages (from-to)212-222
    Number of pages11
    JournalPlant Physiology
    Volume148
    Issue number1
    DOIs
    Publication statusPublished - Sep 2008

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