Application of epifluorescence scanning for monitoring the efficacy of protein removal by RF gas-plasma decontamination

Helen C. Baxter, Patricia R. Richardson, Gaynor A. Campbell, Valeri I. Koalev, Robert Maier, James S. Barton, Anita C. Jones, Greg Delarge, Mark Casey, Robert L. Baxter

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Abstract

The development of methods for measuring the efficiency of gas-plasma decontamination has lagged far behind application. An approach to measuring the efficiency of protein removal from solid surfaces using fluorescein-labelled bovine serum albumin and epifluorescence scanning (EFSCAN) is described. A method for fluorescently labelling proteins, which are adsorbed and denatured on metal surfaces, has been developed. Both approaches have been used to evaluate the efficiency of radio frequency (RF) gas-plasma decontamination protocols. Examples with 'real' surgical instruments demonstrate that an argon-oxygen RF gas-plasma treatment can routinely reduce the protein load by about three orders of magnitude beyond that achieved by current decontamination methods. © IOP Publishing Ltd and Deutsche Physikalische Gesellschaft.

Original languageEnglish
Article number115028
JournalNew Journal of Physics
Volume11
Issue numberNovember 2009
DOIs
Publication statusPublished - 26 Nov 2009

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    Baxter, H. C., Richardson, P. R., Campbell, G. A., Koalev, V. I., Maier, R., Barton, J. S., Jones, A. C., Delarge, G., Casey, M., & Baxter, R. L. (2009). Application of epifluorescence scanning for monitoring the efficacy of protein removal by RF gas-plasma decontamination. New Journal of Physics, 11(November 2009), [115028]. https://doi.org/10.1088/1367-2630/11/11/115028