Antimicrobial Properties of Gallium(III)-and Iron(III)-Loaded Polysaccharides Affecting the Growth of Escherichia coli, Staphylococcus aureus, and Pseudomonas aeruginosa, in Vitro

Mark G. Best, Cassilda Cunha-Reis, Alexey Y. Ganin, Aureliana Sousa, Jenna Johnston, Ana L. Oliveira, David G. E. Smith, Humphrey H. P. Yiu, Ian R. Cooper

Research output: Contribution to journalArticlepeer-review

Abstract

Antimicrobial resistance (AMR) has become a global concern as many bacterial species have developed resistance to commonly prescribed antibiotics, making them ineffective to treatments. One type of antibiotics, gallium(III) compounds, stands out as possible candidates due to their unique "Trojan horse"mechanism to tackle bacterial growth, by substituting iron(III) in the metabolic cycles of bacteria. In this study, we tested three polysaccharides (carboxymethyl cellulose (CMC), alginate, and pectin) as the binding and delivery agent for gallium on three bacteria (Pseudomonas aeruginosa, Escherichia coli, and Staphylococcus aureus) with a potential bioresponsive delivery mode. Two types of analysis on bacterial growth (minimum inhibitory concentrations (MIC) and minimum bactericidal concentrations (MBC)) were carried out while iron(III)-loaded polysaccharide samples were also tested for comparison. The results suggested that gallium showed an improved inhibitory activity on bacterial growth, in particular gallium(III)-loaded carboxymethyl cellulose (Ga-CMC) sample showing an inhibiting effect on growth for all three tested bacteria. At the MIC for all three bacteria, Ga-CMC showed no cytotoxicity effect on human dermal neonatal fibroblasts (HDNF). Therefore, these bioresponsive gallium(III) polysaccharide compounds show significant potential to be developed as the next-generation antibacterial agents with controlled release capability.

Original languageEnglish
Pages (from-to)7589-7597
Number of pages9
JournalACS Applied Bio Materials
Volume3
Issue number11
Early online date12 Oct 2020
DOIs
Publication statusPublished - 16 Nov 2020

Keywords

  • anaerobic
  • antibacterial
  • antibiotics
  • carbohydrates
  • gallium

ASJC Scopus subject areas

  • Biomaterials
  • Chemistry(all)
  • Biomedical Engineering
  • Biochemistry, medical

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