Antimicrobial and biofilm-disrupting nanostructured TiO2 coating demonstrating photoactivity and dark activity

Alibe Wasa, Johann G. Land, Rukmini Gorthy, Susan Krumdieck, Catherine Bishop, William Godsoe, Jack A. Heinemann

Research output: Contribution to journalArticlepeer-review

8 Citations (Scopus)


Antimicrobial materials are tools used to reduce the transmission of infectious microorganisms. Photo-illuminated titania (TiO2) is a known antimicrobial material. Used as a coating on door handles and similar surfaces, it may reduce viability and colonization by pathogens and limit their spread. We tested the survival of Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus and Saccharomyces cerevisiae on a nano-structured TiO2-based thin film, called 'NsARC', and on stainless steel under a variety of light wavelengths and intensities. There was significantly less survival (P <0.001) of all the organisms tested on NsARC compared to inert uncoated stainless steel under all conditions. NsARC was active in the dark and possible mechanisms for this are suggested. NsARC inhibited biofilm formation as confirmed by scanning electron microscopy. These results suggest that NsARC can be used as a self-cleaning and self-sterilizing antimicrobial surface coating for the prevention and reduction in the spread of potentially infectious microbes.

Original languageEnglish
Article numberfnab039
JournalFEMS Microbiology Letters
Issue number7
Early online date17 Apr 2021
Publication statusPublished - Apr 2021


  • antibiofilm activity
  • antimicrobial activity
  • dark-active photocatalyst
  • nanostructured anatase rutile carbon
  • scanning electron microscopy

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology
  • Genetics


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