Antibody responses of rainbow trout (Oncorhynchus mykiss, Walbaum) to live Aeromonas hydrophila as assessed by various antigen preparations

P. N. Loghothetis, B. Austin

    Research output: Contribution to journalArticle

    Abstract

    Sera from rainbow trout (Oncorhynchus mykiss, Walbaum), which had been immunised with live cells of Aeromonas hydrophila, caused strong agglutination of formalised and live cells of A. hydrophila. In contrast, lower titres were recorded with sheared and pasteurised cells. Extremely weak agglutination occurred with boiled or lipopolysaccharide (LPS) deficient cells. By means of enzyme linked immunosorbent assays (ELISA), it was revealed that trout antiserum reacted with LPS, extracellular products (ECP), exopolysaccharides (EPS), and subcellular components derived from cells which had been pasteurised, sheared, boiled or extracted with EDTA and/or guanidine. It was concluded that LPS is the major antigenic component of whole cells, whereas EPS and a haemolytic component of the ECP could have induced an antibody response in the fish. © 1996 Academic Press Limited.

    Original languageEnglish
    Pages (from-to)455-464
    Number of pages10
    JournalFish and Shellfish Immunology
    Volume6
    Issue number6
    DOIs
    Publication statusPublished - Aug 1996

    Fingerprint

    Aeromonas hydrophila
    Oncorhynchus mykiss
    antigens
    antibodies
    lipopolysaccharides
    exopolysaccharides
    agglutination
    cells
    guanidines
    blood serum
    trout
    antiserum
    enzyme-linked immunosorbent assay
    fish

    Keywords

    • Aeromonas hydrophila
    • Antigens
    • Exopolysaccharide
    • Exotoxins
    • Lipopolysaccharide
    • Rainbow trout

    Cite this

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    abstract = "Sera from rainbow trout (Oncorhynchus mykiss, Walbaum), which had been immunised with live cells of Aeromonas hydrophila, caused strong agglutination of formalised and live cells of A. hydrophila. In contrast, lower titres were recorded with sheared and pasteurised cells. Extremely weak agglutination occurred with boiled or lipopolysaccharide (LPS) deficient cells. By means of enzyme linked immunosorbent assays (ELISA), it was revealed that trout antiserum reacted with LPS, extracellular products (ECP), exopolysaccharides (EPS), and subcellular components derived from cells which had been pasteurised, sheared, boiled or extracted with EDTA and/or guanidine. It was concluded that LPS is the major antigenic component of whole cells, whereas EPS and a haemolytic component of the ECP could have induced an antibody response in the fish. {\circledC} 1996 Academic Press Limited.",
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    Antibody responses of rainbow trout (Oncorhynchus mykiss, Walbaum) to live Aeromonas hydrophila as assessed by various antigen preparations. / Loghothetis, P. N.; Austin, B.

    In: Fish and Shellfish Immunology, Vol. 6, No. 6, 08.1996, p. 455-464.

    Research output: Contribution to journalArticle

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    AU - Austin, B.

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