Anti-human immunodeficiency virus type 1 (HIV-1) activity of lectins from Narcissus species

S. Lopez, J. Bastida, F. Viladomat, M. Armand-Ugon, J. Este, C. Codina, D. Stewart

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    21 Citations (Scopus)

    Abstract

    Mannose-specific lectins (MSLs) were isolated from bulbs of fifteen wild Narcissus species growing in Spain and assayed for their HIV-1 infection inhibitory activity in MT-4 cells and compared to the Narcissus pseudonarcissus agglutinin (NPA), the commercially available MSL obtained from daffodils. Almost all the tested MSLs were found to be active, showing EC50 values (µg/mL) similar to that of NPA, with some being comparable to those obtained with dextran sulfate without significant cytotoxicity. However, on a molar basis almost all of the MSLs tested exhibited lower EC50 values than dextran sulfate whilst six MSLs had values lower than AZT. The most efficacious anti-HIV-1 activity was exhibited by the Narcissus tortifolious MSL, which was 10- (µg/mL) and 100- (molarity) fold more potent than dextran sulfate. Significantly, although this MSL was 15-fold less potent than AZT in terms of quantity (µg/mL), it was 68-fold more potent on a molar basis. The antiviral indices, a ratio of the concentrations that produce cytotoxicity and HIV-1 replication, were calculated and three of the MSLs, N. confusus, N. leonensis and N. tortifolius reported 1.5-, 2- and 8.5-fold greater AI values than dextran sulfate or AZT. Comparison of MSL haemagglutination activities (HAA) to their anti-HIV-1 activities showed that there was no significant correlation. It was suggested that this may be due to a dissociation between both activities as a consequence of multiple isolectin composition.
    Copyright © 2003, Rights Managed by Georg Thieme Verlag KG Stuttgart • New York

    Original languageEnglish
    Pages (from-to)109-112
    Number of pages4
    JournalPlanta Medica
    Volume69
    Issue number2
    DOIs
    Publication statusPublished - 2003

    Keywords

    • Anti-HIV-1 activity
    • mannose-specific lentins
    • Narcissus
    • amaryllidaceae
    • cytotoxicity

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    Lopez, S., Bastida, J., Viladomat, F., Armand-Ugon, M., Este, J., Codina, C., & Stewart, D. (2003). Anti-human immunodeficiency virus type 1 (HIV-1) activity of lectins from Narcissus species. Planta Medica, 69(2), 109-112. https://doi.org/10.1055/s-2003-37715