Analysis of the elements of catabolite repression in Clostridium acetobutylicum ATCC 824

Martin Tangney, Anne Galinier, Josef Deutscher, Wilfrid J. Mitchell

Research output: Contribution to journalArticlepeer-review

38 Citations (Scopus)

Abstract

The ptsH gene, encoding the phosphotransferase protein HPr, from Clostridium acetobutylicum ATCC 824 was identified from the genome sequence, cloned and shown to complement a ptsH mutant of Escherichia coli. The deduced protein sequence shares significant homology with HPr proteins from other low-GC gram-positive bacteria, although the highly conserved sequence surrounding the Ser-46 phosphorylation site is not well preserved in the clostridial protein. Nevertheless, the HPr was phosphorylated in an ATP-dependent manner in cell-free extracts of C. acetobutylicum. Furthermore, purified His-tagged HPr from Bacillus subtilis was also a substrate for the clostridial HPr kinase/phosphorylase. This phosphorylation reaction is a key step in the mechanism of carbon catabolite repression proposed to operate in B. subtilisand other low-GC gram-positive bacteria. Putative genes encoding the HPr kinase/phosphorylase and the other element of this model, namely the catabolite control protein CcpA, were identified from the C. acetobutylicum genome sequence, suggesting that a similar mechanism of carbon catabolite repression may operate in this industrially important organism. Copyright © 2003 S. Karger AG, Basel.

Original languageEnglish
Pages (from-to)6-11
Number of pages6
JournalJournal of Molecular Microbiology and Biotechnology
Volume6
Issue number1
DOIs
Publication statusPublished - 2003

Keywords

  • CcpA
  • HPr
  • Phosphotransferase system
  • Protein kinase

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