Analysis of Parasitic Protozoa at the Single-cell Level using Microfluidic Impedance Cytometry

J. S. McGrath, C. Honrado, D. Spencer, B. Horton, H. L. Bridle, H. Morgan

Research output: Contribution to journalArticle

31 Citations (Scopus)
54 Downloads (Pure)

Abstract

At present, there are few technologies which enable the detection, identification and viability analysis of protozoan pathogens including Cryptosporidium and/or Giardia at the single (oo)cyst level. We report the use of Microfluidic Impedance Cytometry (MIC) to characterise the AC electrical (impedance) properties of single parasites and demonstrate rapid discrimination based on viability and species. Specifically, MIC was used to identify live and inactive C. parvum oocysts with over 90% certainty, whilst also detecting damaged and/or excysted oocysts. Furthermore, discrimination of Cryptosporidium parvum, Cryptosporidium muris and Giardia lamblia, with over 92% certainty was achieved. Enumeration and identification of (oo)cysts can be achieved in a few minutes, which offers a reduction in identification time and labour demands when compared to existing detection methods.

Original languageEnglish
Article number2601
JournalScientific Reports
Volume7
DOIs
Publication statusPublished - 1 Jun 2017

ASJC Scopus subject areas

  • General

Fingerprint Dive into the research topics of 'Analysis of Parasitic Protozoa at the Single-cell Level using Microfluidic Impedance Cytometry'. Together they form a unique fingerprint.

  • Cite this