Analysis for paraquat by second- and fourth-derivative spectroscopy

Anthony F Fell, D. R. Jarvie, M. J. Stewart

Research output: Contribution to journalArticle

Abstract

We describe simple, precise methods, based on second- and fourth-derivative spectroscopy, of analysis for paraquat in serum, plasma, or dialysis fluid. Plasma paraquat, after ion-pair extraction and dithionite reduction, yields a sharp, stable absorption peak at 396 nm. Derivative spectroscopy confers enhanced sensitivity (detection limit, 5 µg/L) and improved rejection of spectroscopic interference by diquat, hemolysis, bilirubin, or lipemia. Derivative peak amplitudes are linearly related to paraquat dichloride concentration from 10 to 1000 µg/L in plasma, and the methods are precise (CV 2.7-5.2% for 50 µg/L concentration) and accurate. Results of derivative assays for 18 cases of paraquat poisoning correlate well with those by radioimmunoassay (r=0.999). Derivative spectroscopy enables monitoring of paraquat concentrations in the plasma of poisoned patients during treatment by hemoperfusion or hemodialysis. A rapid assay developed for dialysis fluid (detection limit, 2 µg/L) permits the efficacy of hemodialysis to be routinely assessed.

Original languageEnglish
Pages (from-to)286-292
Number of pages7
JournalClinical Chemistry
Volume27
Issue number2
Publication statusPublished - 1981

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    Fell, A. F., Jarvie, D. R., & Stewart, M. J. (1981). Analysis for paraquat by second- and fourth-derivative spectroscopy. Clinical Chemistry, 27(2), 286-292.