An octaheme c-type cytochrome from Shewanella oneidensis can reduce nitrite and hydroxylamine

Sally Atkinson, Christopher Mowat, Graeme Reid, Stephen Chapman

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    Abstract

    A c-type cytochrome from Shewanella oneidensis MR-1, containing eight hemes, has been previously designated as an octaheme tetrathionate reductase (OTR). The structure of OTR revealed that the active site contains an unusual lysine-ligated heme, despite the presence of a CXXCH motif in the sequence that would predict histidine ligation. This lysine ligation has been previously observed only in the pentaheme nitrite reductases, suggesting that OTR may have a possible role in nitrite reduction. We have now shown that OTR is an efficient nitrite and hydroxylamine reductase and that ammonium ion is the product. These results indicate that OTR may have a role in the biological nitrogen cycle.

    Original languageUndefined/Unknown
    Pages (from-to)3805-3808
    Number of pages4
    JournalFederation of European Biochemical Societies Letters
    Volume581
    Issue number20
    DOIs
    Publication statusPublished - 7 Aug 2007

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