An improved selective isolation medium for the recovery of Listeria monocytogenes from smoked fish

A. A N Neamatallah, S. J. Dewar, B. Austin

    Research output: Contribution to journalArticle

    4 Citations (Scopus)

    Abstract

    Aims: The aim of this study was to improve the selective isolation of Listeria monocytogenes from smoked haddock fillets. Methods and Results: Listeria selective agar (LSA) - Oxford formulation was supplemented with 25 µg ml-1 of colistin sulphate and 30 µg ml-1 of nalidixic acid. Inocula from four smoked haddock fillets produced colonies (approx. 2-13 bacteria g-1), identified as L. monocytogenes, on LSA supplemented with antimicrobial compounds (MLSA). Moreover, there was only negligible evidence of bacteria which were not L. monocytogenes on MLSA. In contrast, LSA supported dense bacterial growth, which was not equated with L. monocytogenes. Significance and Impact of the Study: The modified medium permitted the recovery of L. monocytogenes from smoked haddock fillets and reduced the growth of contaminating bacteria.

    Original languageEnglish
    Pages (from-to)230-233
    Number of pages4
    JournalLetters in Applied Microbiology
    Volume36
    Issue number4
    Publication statusPublished - 2003

    Keywords

    • Listeria monocytogenes
    • Selective isolation
    • Smoked haddock

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