An improved selective isolation medium for the recovery of Listeria monocytogenes from smoked fish

A. A N Neamatallah; S. J. Dewar; B. Austin

An improved selective isolation medium for the recovery of Listeria monocytogenes from smoked fish

A. A N Neamatallah, S. J. Dewar, B. Austin

Research output: Contribution to journal › Article › peer-review

Abstract

Aims: The aim of this study was to improve the selective isolation of Listeria monocytogenes from smoked haddock fillets. Methods and Results: Listeria selective agar (LSA) - Oxford formulation was supplemented with 25 µg ml^-1 of colistin sulphate and 30 µg ml^-1 of nalidixic acid. Inocula from four smoked haddock fillets produced colonies (approx. 2-13 bacteria g^-1), identified as L. monocytogenes, on LSA supplemented with antimicrobial compounds (MLSA). Moreover, there was only negligible evidence of bacteria which were not L. monocytogenes on MLSA. In contrast, LSA supported dense bacterial growth, which was not equated with L. monocytogenes. Significance and Impact of the Study: The modified medium permitted the recovery of L. monocytogenes from smoked haddock fillets and reduced the growth of contaminating bacteria.

Original language	English
Pages (from-to)	230-233
Number of pages	4
Journal	Letters in Applied Microbiology
Volume	36
Issue number	4
Publication status	Published - 2003

Keywords

Listeria monocytogenes
Selective isolation
Smoked haddock

Cite this

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title = "An improved selective isolation medium for the recovery of Listeria monocytogenes from smoked fish",

abstract = "Aims: The aim of this study was to improve the selective isolation of Listeria monocytogenes from smoked haddock fillets. Methods and Results: Listeria selective agar (LSA) - Oxford formulation was supplemented with 25 µg ml-1 of colistin sulphate and 30 µg ml-1 of nalidixic acid. Inocula from four smoked haddock fillets produced colonies (approx. 2-13 bacteria g-1), identified as L. monocytogenes, on LSA supplemented with antimicrobial compounds (MLSA). Moreover, there was only negligible evidence of bacteria which were not L. monocytogenes on MLSA. In contrast, LSA supported dense bacterial growth, which was not equated with L. monocytogenes. Significance and Impact of the Study: The modified medium permitted the recovery of L. monocytogenes from smoked haddock fillets and reduced the growth of contaminating bacteria.",

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AU - Neamatallah, A. A N

AU - Dewar, S. J.

AU - Austin, B.

PY - 2003

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N2 - Aims: The aim of this study was to improve the selective isolation of Listeria monocytogenes from smoked haddock fillets. Methods and Results: Listeria selective agar (LSA) - Oxford formulation was supplemented with 25 µg ml-1 of colistin sulphate and 30 µg ml-1 of nalidixic acid. Inocula from four smoked haddock fillets produced colonies (approx. 2-13 bacteria g-1), identified as L. monocytogenes, on LSA supplemented with antimicrobial compounds (MLSA). Moreover, there was only negligible evidence of bacteria which were not L. monocytogenes on MLSA. In contrast, LSA supported dense bacterial growth, which was not equated with L. monocytogenes. Significance and Impact of the Study: The modified medium permitted the recovery of L. monocytogenes from smoked haddock fillets and reduced the growth of contaminating bacteria.

AB - Aims: The aim of this study was to improve the selective isolation of Listeria monocytogenes from smoked haddock fillets. Methods and Results: Listeria selective agar (LSA) - Oxford formulation was supplemented with 25 µg ml-1 of colistin sulphate and 30 µg ml-1 of nalidixic acid. Inocula from four smoked haddock fillets produced colonies (approx. 2-13 bacteria g-1), identified as L. monocytogenes, on LSA supplemented with antimicrobial compounds (MLSA). Moreover, there was only negligible evidence of bacteria which were not L. monocytogenes on MLSA. In contrast, LSA supported dense bacterial growth, which was not equated with L. monocytogenes. Significance and Impact of the Study: The modified medium permitted the recovery of L. monocytogenes from smoked haddock fillets and reduced the growth of contaminating bacteria.

KW - Listeria monocytogenes

KW - Selective isolation

KW - Smoked haddock

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M3 - Article

SN - 1472-765X

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JO - Letters in Applied Microbiology

JF - Letters in Applied Microbiology

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ER -

An improved selective isolation medium for the recovery of Listeria monocytogenes from smoked fish

Abstract

Keywords

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