The problem of eluent optimisation in reversed-phase liquid chromatography is a complex diagnostic situation, amenable to an expert system approach. Such a system has been developed in microProlog, which uses a gradient elution experiment to determine the appropriate initial solvent strength for a given separation, followed by response-surface modelling using an iterative lattice method to determine the mobile phase composition for optimum resolution. Spectral information from a diode array detector is used to track the retention position of each component as the mobile phase composition is varied. Peak homogeneity is assessed by a number of independent modules, the output from which is utilised by the expert system to validate the model constructed by the optimisation procedure. © 1987 Friedr. Vieweg & Sohn Verlagsgesellschaft mbH.
- Expert system
- Mobile phase optimisation
- Peak homogeneity validation
- Photodiode array detection
- Reversed-phase liquid chromatography