An enzymic assay of l-arabinose, using β-d-galactose dehydrogenase

Its application in the assay of α-l-arabinofuranosidase

James Melrose, Robert J. Sturgeon

    Research output: Contribution to journalArticle

    Abstract

    A new spectrophotometric method has been developed for the quantitative determination of l-arabinose, using the NAD-dependent ß-d-galactose dehydrogenase from Pseudomonas fluorescens. This method has been applied to the determination of l-arabinose produced after degradation of l-arabino-d-xylan by a-l-arabinofuranosidase from Aspergillus fumigatus, and provides the basis of a sensitive, accurate, and specific method for assay of this enzyme which is technically straightforward to perform. © 1983.

    Original languageEnglish
    Pages (from-to)247-253
    Number of pages7
    JournalCarbohydrate Research
    Volume118
    Issue numberC
    Publication statusPublished - 16 Jul 1983

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    galactose dehydrogenase
    Arabinose
    Assays
    Xylans
    Aspergillus
    NAD
    Degradation
    Enzymes

    Cite this

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    title = "An enzymic assay of l-arabinose, using β-d-galactose dehydrogenase: Its application in the assay of α-l-arabinofuranosidase",
    abstract = "A new spectrophotometric method has been developed for the quantitative determination of l-arabinose, using the NAD-dependent {\ss}-d-galactose dehydrogenase from Pseudomonas fluorescens. This method has been applied to the determination of l-arabinose produced after degradation of l-arabino-d-xylan by a-l-arabinofuranosidase from Aspergillus fumigatus, and provides the basis of a sensitive, accurate, and specific method for assay of this enzyme which is technically straightforward to perform. {\circledC} 1983.",
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    An enzymic assay of l-arabinose, using β-d-galactose dehydrogenase : Its application in the assay of α-l-arabinofuranosidase. / Melrose, James; Sturgeon, Robert J.

    In: Carbohydrate Research, Vol. 118, No. C, 16.07.1983, p. 247-253.

    Research output: Contribution to journalArticle

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    T2 - Its application in the assay of α-l-arabinofuranosidase

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    AB - A new spectrophotometric method has been developed for the quantitative determination of l-arabinose, using the NAD-dependent ß-d-galactose dehydrogenase from Pseudomonas fluorescens. This method has been applied to the determination of l-arabinose produced after degradation of l-arabino-d-xylan by a-l-arabinofuranosidase from Aspergillus fumigatus, and provides the basis of a sensitive, accurate, and specific method for assay of this enzyme which is technically straightforward to perform. © 1983.

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