An enzymic assay of l-arabinose, using β-d-galactose dehydrogenase: Its application in the assay of α-l-arabinofuranosidase

James Melrose; Robert J. Sturgeon

An enzymic assay of l-arabinose, using β-d-galactose dehydrogenase: Its application in the assay of α-l-arabinofuranosidase

James Melrose, Robert J. Sturgeon

Research output: Contribution to journal › Article › peer-review

Abstract

A new spectrophotometric method has been developed for the quantitative determination of l-arabinose, using the NAD-dependent ß-d-galactose dehydrogenase from Pseudomonas fluorescens. This method has been applied to the determination of l-arabinose produced after degradation of l-arabino-d-xylan by a-l-arabinofuranosidase from Aspergillus fumigatus, and provides the basis of a sensitive, accurate, and specific method for assay of this enzyme which is technically straightforward to perform. © 1983.

Original language	English
Pages (from-to)	247-253
Number of pages	7
Journal	Carbohydrate Research
Volume	118
Issue number	C
Publication status	Published - 16 Jul 1983

Cite this

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abstract = "A new spectrophotometric method has been developed for the quantitative determination of l-arabinose, using the NAD-dependent {\ss}-d-galactose dehydrogenase from Pseudomonas fluorescens. This method has been applied to the determination of l-arabinose produced after degradation of l-arabino-d-xylan by a-l-arabinofuranosidase from Aspergillus fumigatus, and provides the basis of a sensitive, accurate, and specific method for assay of this enzyme which is technically straightforward to perform. {\textcopyright} 1983.",

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N2 - A new spectrophotometric method has been developed for the quantitative determination of l-arabinose, using the NAD-dependent ß-d-galactose dehydrogenase from Pseudomonas fluorescens. This method has been applied to the determination of l-arabinose produced after degradation of l-arabino-d-xylan by a-l-arabinofuranosidase from Aspergillus fumigatus, and provides the basis of a sensitive, accurate, and specific method for assay of this enzyme which is technically straightforward to perform. © 1983.

AB - A new spectrophotometric method has been developed for the quantitative determination of l-arabinose, using the NAD-dependent ß-d-galactose dehydrogenase from Pseudomonas fluorescens. This method has been applied to the determination of l-arabinose produced after degradation of l-arabino-d-xylan by a-l-arabinofuranosidase from Aspergillus fumigatus, and provides the basis of a sensitive, accurate, and specific method for assay of this enzyme which is technically straightforward to perform. © 1983.

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JO - Carbohydrate Research

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ER -

An enzymic assay of l-arabinose, using β-d-galactose dehydrogenase: Its application in the assay of α-l-arabinofuranosidase

Abstract

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