An enzyme-linked immunosorbent assay (ELISA) for the detection of Vibrio harveyi in penaeid shrimp and water

P. A W Robertson, H. S. Xu, B. Austin

    Research output: Contribution to journalArticle

    Abstract

    Plate and dipstick enzyme-linked immunosorbent assays (ELISA) were developed for the rapid detection of Vibrio harveyi from penaeid shrimp and water. The ELISA, which incorporated a polyclonal antiserum produced in a female New Zealand white rabbit, detected 105 cells of V. harveyi/ml. Also, the systems detected V. harveyi in water from Chinese shrimp hatcheries. The systems permitted the recognition of a wide range of V. harveyi isolates, but not those of other taxa. Western blot analysis of bacterial outer membrane proteins (OMP) indicated that a wide epitope was recognised, with many immunoreactive bands in common between isolates of V. harveyi. Copyright (C) 1998 Elsevier Science B.V.

    Original languageEnglish
    Pages (from-to)31-39
    Number of pages9
    JournalJournal of Microbiological Methods
    Volume34
    Issue number1
    DOIs
    Publication statusPublished - 1 Sep 1998

    Fingerprint

    Vibrio harveyi
    Penaeidae
    enzyme-linked immunosorbent assay
    water
    New Zealand White rabbit
    outer membrane proteins
    hatcheries
    epitopes
    antiserum
    Western blotting
    shrimp
    cells

    Keywords

    • ELISA
    • Penaeids
    • Shrimps
    • Vibrio harveyi
    • Western blots

    Cite this

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    abstract = "Plate and dipstick enzyme-linked immunosorbent assays (ELISA) were developed for the rapid detection of Vibrio harveyi from penaeid shrimp and water. The ELISA, which incorporated a polyclonal antiserum produced in a female New Zealand white rabbit, detected 105 cells of V. harveyi/ml. Also, the systems detected V. harveyi in water from Chinese shrimp hatcheries. The systems permitted the recognition of a wide range of V. harveyi isolates, but not those of other taxa. Western blot analysis of bacterial outer membrane proteins (OMP) indicated that a wide epitope was recognised, with many immunoreactive bands in common between isolates of V. harveyi. Copyright (C) 1998 Elsevier Science B.V.",
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    An enzyme-linked immunosorbent assay (ELISA) for the detection of Vibrio harveyi in penaeid shrimp and water. / Robertson, P. A W; Xu, H. S.; Austin, B.

    In: Journal of Microbiological Methods, Vol. 34, No. 1, 01.09.1998, p. 31-39.

    Research output: Contribution to journalArticle

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