An enzyme-linked immunosorbent assay (ELISA) for the detection of Vibrio harveyi in penaeid shrimp and water

P. A W Robertson; H. S. Xu; B. Austin

doi:10.1016/S0167-7012(98)00066-9

An enzyme-linked immunosorbent assay (ELISA) for the detection of Vibrio harveyi in penaeid shrimp and water

P. A W Robertson, H. S. Xu, B. Austin

Research output: Contribution to journal › Article › peer-review

19 Citations (Scopus)

Abstract

Plate and dipstick enzyme-linked immunosorbent assays (ELISA) were developed for the rapid detection of Vibrio harveyi from penaeid shrimp and water. The ELISA, which incorporated a polyclonal antiserum produced in a female New Zealand white rabbit, detected 10⁵ cells of V. harveyi/ml. Also, the systems detected V. harveyi in water from Chinese shrimp hatcheries. The systems permitted the recognition of a wide range of V. harveyi isolates, but not those of other taxa. Western blot analysis of bacterial outer membrane proteins (OMP) indicated that a wide epitope was recognised, with many immunoreactive bands in common between isolates of V. harveyi. Copyright (C) 1998 Elsevier Science B.V.

Original language	English
Pages (from-to)	31-39
Number of pages	9
Journal	Journal of Microbiological Methods
Volume	34
Issue number	1
DOIs	https://doi.org/10.1016/S0167-7012(98)00066-9
Publication status	Published - 1 Sept 1998

Keywords

ELISA
Penaeids
Shrimps
Vibrio harveyi
Western blots

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10.1016/S0167-7012(98)00066-9

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AB - Plate and dipstick enzyme-linked immunosorbent assays (ELISA) were developed for the rapid detection of Vibrio harveyi from penaeid shrimp and water. The ELISA, which incorporated a polyclonal antiserum produced in a female New Zealand white rabbit, detected 105 cells of V. harveyi/ml. Also, the systems detected V. harveyi in water from Chinese shrimp hatcheries. The systems permitted the recognition of a wide range of V. harveyi isolates, but not those of other taxa. Western blot analysis of bacterial outer membrane proteins (OMP) indicated that a wide epitope was recognised, with many immunoreactive bands in common between isolates of V. harveyi. Copyright (C) 1998 Elsevier Science B.V.

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An enzyme-linked immunosorbent assay (ELISA) for the detection of Vibrio harveyi in penaeid shrimp and water

Abstract

Keywords

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