Plate and dipstick enzyme-linked immunosorbent assays (ELISA) were developed for the rapid detection of Vibrio harveyi from penaeid shrimp and water. The ELISA, which incorporated a polyclonal antiserum produced in a female New Zealand white rabbit, detected 105 cells of V. harveyi/ml. Also, the systems detected V. harveyi in water from Chinese shrimp hatcheries. The systems permitted the recognition of a wide range of V. harveyi isolates, but not those of other taxa. Western blot analysis of bacterial outer membrane proteins (OMP) indicated that a wide epitope was recognised, with many immunoreactive bands in common between isolates of V. harveyi. Copyright (C) 1998 Elsevier Science B.V.
- Vibrio harveyi
- Western blots