An Antarctic molluscan biomineralisation tool-kit

Victoria Anne Sleight, Benjamin Marie, Daniel J. Jackson, Elizabeth Dyrynda, Arul Marie, Melody S. Clark

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7 Citations (Scopus)
30 Downloads (Pure)

Abstract

The Antarctic clam Laternula elliptica lives almost permanently below 0 °C and therefore is a valuable and tractable model to study the mechanisms of biomineralisation in cold water. The present study employed a multidisciplinary approach using histology, immunohistochemistry, electron microscopy, proteomics and gene expression to investigate this process. Thirty seven proteins were identified via proteomic extraction of the nacreous shell layer, including two not previously found in nacre; a novel T-rich Mucin-like protein and a Zinc-dependent metalloprotease. In situ hybridisation of seven candidate biomineralisation genes revealed discrete spatial expression patterns within the mantle tissue, hinting at modular organisation, which is also observed in the mantle tissues of other molluscs. All seven of these biomineralisation candidates displayed evidence of multifunctionality and strong association with vesicles, which are potentially involved in shell secretion in this species.

Original languageEnglish
Article number36978
JournalScientific Reports
Volume6
DOIs
Publication statusPublished - 11 Nov 2016

ASJC Scopus subject areas

  • General

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    Sleight, V. A., Marie, B., Jackson, D. J., Dyrynda, E., Marie, A., & Clark, M. S. (2016). An Antarctic molluscan biomineralisation tool-kit. Scientific Reports, 6, [36978]. https://doi.org/10.1038/srep36978