TY - JOUR
T1 - Affinity chromatography of sialoglycoproteins, utilising the interaction of serotonin with N-acetylneuraminic acid and its derivatives
AU - Sturgeon, Robert J.
AU - Sturgeon, Catharine M.
PY - 1982/5/16
Y1 - 1982/5/16
N2 - Serotonin, immobilised on Sepharose 4B, has been used to study the affinity chromatography of neuraminic acid and its derivatives. Free N-acetylneuraminic acid and oligosaccharides, polysaccharides, and glycoproteins containing that sugar are specifically bound to the columns. Removal of neuraminic acid from sialoglyco-conjugates, or modification of the neuraminic acid residues by periodate oxidation, abolishes their ability to bind to the ligand. The presence of the N-acetyl group, but not the N-glycolyl group, and the integrity of the side chain (C-7-C-9) of the neuraminic acid are essential for binding to serotonin. © 1982.
AB - Serotonin, immobilised on Sepharose 4B, has been used to study the affinity chromatography of neuraminic acid and its derivatives. Free N-acetylneuraminic acid and oligosaccharides, polysaccharides, and glycoproteins containing that sugar are specifically bound to the columns. Removal of neuraminic acid from sialoglyco-conjugates, or modification of the neuraminic acid residues by periodate oxidation, abolishes their ability to bind to the ligand. The presence of the N-acetyl group, but not the N-glycolyl group, and the integrity of the side chain (C-7-C-9) of the neuraminic acid are essential for binding to serotonin. © 1982.
UR - http://www.scopus.com/inward/record.url?scp=0020484990&partnerID=8YFLogxK
M3 - Article
SN - 0008-6215
VL - 103
SP - 213
EP - 219
JO - Carbohydrate Research
JF - Carbohydrate Research
IS - 2
ER -