Affinity chromatography of sialoglycoproteins, utilising the interaction of serotonin with N-acetylneuraminic acid and its derivatives

Robert J. Sturgeon, Catharine M. Sturgeon

    Research output: Contribution to journalArticle

    Abstract

    Serotonin, immobilised on Sepharose 4B, has been used to study the affinity chromatography of neuraminic acid and its derivatives. Free N-acetylneuraminic acid and oligosaccharides, polysaccharides, and glycoproteins containing that sugar are specifically bound to the columns. Removal of neuraminic acid from sialoglyco-conjugates, or modification of the neuraminic acid residues by periodate oxidation, abolishes their ability to bind to the ligand. The presence of the N-acetyl group, but not the N-glycolyl group, and the integrity of the side chain (C-7-C-9) of the neuraminic acid are essential for binding to serotonin. © 1982.

    Original languageEnglish
    Pages (from-to)213-219
    Number of pages7
    JournalCarbohydrate Research
    Volume103
    Issue number2
    Publication statusPublished - 16 May 1982

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    Neuraminic Acids
    Sialoglycoproteins
    Affinity chromatography
    N-Acetylneuraminic Acid
    Serotonin
    Derivatives
    Oligosaccharides
    Sugars
    Sepharose
    Polysaccharides
    Glycoproteins
    Ligands
    Oxidation

    Cite this

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    Affinity chromatography of sialoglycoproteins, utilising the interaction of serotonin with N-acetylneuraminic acid and its derivatives. / Sturgeon, Robert J.; Sturgeon, Catharine M.

    In: Carbohydrate Research, Vol. 103, No. 2, 16.05.1982, p. 213-219.

    Research output: Contribution to journalArticle

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