Adhesion and migration of cells responding to microtopography

Maruxa Estévez, Elena Martínez, Stephen J Yarwood, Matthew J. Dalby, Josep Samitier

    Research output: Contribution to journalArticle

    21 Citations (Scopus)

    Abstract

    It is known that cells respond strongly to microtopography. However, cellular mechanisms of response are unclear. Here, we study wild-type fibroblasts responding to 25 µm(2) posts and compare their response to that of FAK(-/-) fibroblasts and fibroblasts with PMA treatment to stimulate protein kinase C (PKC) and the small g-protein Rac. FAK knockout cells modulated adhesion number and size in a similar way to cells on topography; that is, they used more, smaller adhesions, but migration was almost completely stalled demonstrating the importance of FAK signaling in contact guidance and adhesion turnover. Little similarity, however, was observed to PKC stimulated cells and cells on the topography. Interestingly, with PKC stimulation the cell nuclei became highly deformable bringing focus on these surfaces to the study of metastasis. Surfaces that aid the study of cellular migration are important in developing understanding of mechanisms of wound healing and repair in aligned tissues such as ligament and tendon.

    Original languageEnglish
    Pages (from-to)1659-1668
    Number of pages10
    JournalJournal of Biomedical Materials Research Part A
    Volume103
    Issue number5
    DOIs
    Publication statusPublished - May 2015

    Keywords

    • Actin Cytoskeleton
    • Animals
    • Cell Adhesion
    • Cell Movement
    • Cell Nucleus
    • Cell Shape
    • Fibroblasts
    • Focal Adhesion Protein-Tyrosine Kinases
    • Focal Adhesions
    • Imaging, Three-Dimensional
    • Interferometry
    • Mice
    • Polymethyl Methacrylate
    • Tetradecanoylphorbol Acetate
    • Vinculin

    Fingerprint Dive into the research topics of 'Adhesion and migration of cells responding to microtopography'. Together they form a unique fingerprint.

  • Cite this