A soil-based microbial biofilm exposed to 2,4-D: bacterial community development and establishment of conjugative plasmid pJP4

T J Aspray, S K Hansen, R G Burns

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    22 Citations (Scopus)

    Abstract

    A soil suspension was used as a source to initiate the development of microbial communities in flow cells irrigated with 2,4-dichlorophenoxyacetic acid (2,4-D) (25 mu g ml(-1)). Culturable bacterial members of the community were identified by 16S rRNA gene sequencing and found to be members of the genera Pseudomonas, Burkholderia, Collimonas and Rhodococcus. A 2,4-D degrading donor strain, Pseudomonas putida SM 1443 (pJP4::gfp), was inoculated into flow cell chambers containing 2-day old biofilm communities. Transfer of pJP4::gfp from the donor to the bacterial community was detectable as GFP fluorescing cells and images were captured using confocal scanning laser microscopy (GFP fluorescence was repressed in the donor due to the presence of a chromosomally located lacl(q) repressor gene). Approximately 5-10 transconjugant microcolonies, 20-40 mu m in diameter, could be seen to develop in each chamber. A 2,4-D degrading transconjugant strain was isolated from the flow cell system belonging to the genus Burkholderia. (C) 2005 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.

    Original languageEnglish
    Pages (from-to)317-327
    Number of pages11
    JournalFEMS Microbiology Ecology
    Volume54
    Issue number2
    DOIs
    Publication statusPublished - 1 Oct 2005

    Keywords

    • green fluorescent protein
    • 2,4-D
    • horizontal plasmid transfer
    • biofilms
    • pJP4
    • 2,4-DICHLOROPHENOXYACETIC ACID 2,4-D
    • MOBILE GENETIC ELEMENTS
    • DEGRADATION PLASMIDS
    • HORIZONTAL TRANSFER
    • CONTAMINATED SOIL
    • BIOAUGMENTATION
    • COMPLEMENTATION
    • TRANSCONJUGANTS
    • PSEUDOMONAS
    • CONSORTIUM

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