A scanning peptide array approach uncovers association sites within the JNK/βarrestin signalling complex

Xiang Li; Ruth MacLeod; Allan J. Dunlop; Helen V. Edwards; Noopur Advant; L. C D Gibson; Nicola M. Devine; Kim M. Brown; David R. Adams; Miles D. Houslay; George S. Baillie

doi:10.1016/j.febslet.2009.09.035

A scanning peptide array approach uncovers association sites within the JNK/βarrestin signalling complex

Xiang Li
, Ruth MacLeod
, Allan J. Dunlop
, Helen V. Edwards
, Noopur Advant
, L. C D Gibson
, Nicola M. Devine
, Kim M. Brown
, David R. Adams
, Miles D. Houslay
, George S. Baillie

School of Engineering & Physical Sciences

Research output: Contribution to journal › Article › peer-review

28 Citations (Scopus)

Abstract

ßarrestins are molecular scaffolds that can bring together three-component mitogen-activated protein kinase signalling modules to promote signal compartmentalisation. We use peptide array technology to define novel interfaces between components within the c-Jun N-terminal kinase (JNK)/ßarrestin signalling complex. We show that ßarrestin 1 and ßarrestin 2 associate with JNK3 via the kinase N-terminal domain in a region that, surprisingly, does not harbour a known 'common docking' motif. In the N-domain and C-terminus of ßarrestin 1 and ßarrestin 2 we identify two novel apoptosis signal-regulating kinase 1 binding sites and in the N-domain of the ßarrestin 1 and ßarrestin 2 we identify a novel MKK4 docking site. Structured summary: MINT-7263196, MINT-7263175: Arrestin beta-2 (uniprotkb:P32121) binds (MI:0407) to ASK1 (uniprotkb:Q99683) by peptide array (MI:0081). MINT-7263136: JNK3 (uniprotkb:P53779) binds (MI:0407) to Arrestin beta-1 (uniprotkb:P49407) by peptide array (MI:0081). MINT-7263161: JNK3 (uniprotkb:P53779) binds (MI:0407) to Arrestin beta-2 (uniprotkb:P32121) by peptide array (MI:0081). MINT-7263304: Arrestin beta-1 (uniprotkb:P49407) physically interacts (MI:0915) with ASK1 (uniprotkb:Q99683) by anti tag coimmunoprecipitation (MI:0007). MINT-7263286: Arrestin beta-2 (uniprotkb:P32121) binds (MI:0407) to MKK4 (uniprotkb:P45985) by peptide array (MI:0081). MINT-7263231, MINT-7263254: Arrestin beta-1 (uniprotkb:P49407) binds (MI:0407) to ASK1 (uniprotkb:Q99683) by peptide array (MI:0081). MINT-7263269: Arrestin beta-1 (uniprotkb:P49407) binds (MI:0407) to MKK4 (uniprotkb:P45985) by peptide array (MI:0081). © 2009 Federation of European Biochemical Societies.

Original language	English
Pages (from-to)	3310-3316
Number of pages	7
Journal	FEBS Letters
Volume	583
Issue number	20
DOIs	https://doi.org/10.1016/j.febslet.2009.09.035
Publication status	Published - 20 Oct 2009

Keywords

Arrestin
ASK1
JNK
MKK4
Peptide array
Scaffold

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10.1016/j.febslet.2009.09.035

Cite this

@article{11d6caeda527498cb61a36c1045a6b21,

title = "A scanning peptide array approach uncovers association sites within the JNK/βarrestin signalling complex",

abstract = "{\ss}arrestins are molecular scaffolds that can bring together three-component mitogen-activated protein kinase signalling modules to promote signal compartmentalisation. We use peptide array technology to define novel interfaces between components within the c-Jun N-terminal kinase (JNK)/{\ss}arrestin signalling complex. We show that {\ss}arrestin 1 and {\ss}arrestin 2 associate with JNK3 via the kinase N-terminal domain in a region that, surprisingly, does not harbour a known 'common docking' motif. In the N-domain and C-terminus of {\ss}arrestin 1 and {\ss}arrestin 2 we identify two novel apoptosis signal-regulating kinase 1 binding sites and in the N-domain of the {\ss}arrestin 1 and {\ss}arrestin 2 we identify a novel MKK4 docking site. Structured summary: MINT-7263196, MINT-7263175: Arrestin beta-2 (uniprotkb:P32121) binds (MI:0407) to ASK1 (uniprotkb:Q99683) by peptide array (MI:0081). MINT-7263136: JNK3 (uniprotkb:P53779) binds (MI:0407) to Arrestin beta-1 (uniprotkb:P49407) by peptide array (MI:0081). MINT-7263161: JNK3 (uniprotkb:P53779) binds (MI:0407) to Arrestin beta-2 (uniprotkb:P32121) by peptide array (MI:0081). MINT-7263304: Arrestin beta-1 (uniprotkb:P49407) physically interacts (MI:0915) with ASK1 (uniprotkb:Q99683) by anti tag coimmunoprecipitation (MI:0007). MINT-7263286: Arrestin beta-2 (uniprotkb:P32121) binds (MI:0407) to MKK4 (uniprotkb:P45985) by peptide array (MI:0081). MINT-7263231, MINT-7263254: Arrestin beta-1 (uniprotkb:P49407) binds (MI:0407) to ASK1 (uniprotkb:Q99683) by peptide array (MI:0081). MINT-7263269: Arrestin beta-1 (uniprotkb:P49407) binds (MI:0407) to MKK4 (uniprotkb:P45985) by peptide array (MI:0081). {\textcopyright} 2009 Federation of European Biochemical Societies.",

keywords = "Arrestin, ASK1, JNK, MKK4, Peptide array, Scaffold",

author = "Xiang Li and Ruth MacLeod and Dunlop, \{Allan J.\} and Edwards, \{Helen V.\} and Noopur Advant and Gibson, \{L. C D\} and Devine, \{Nicola M.\} and Brown, \{Kim M.\} and Adams, \{David R.\} and Houslay, \{Miles D.\} and Baillie, \{George S.\}",

year = "2009",

month = oct,

day = "20",

doi = "10.1016/j.febslet.2009.09.035",

language = "English",

volume = "583",

pages = "3310--3316",

journal = "FEBS Letters",

issn = "0014-5793",

publisher = "John Wiley and Sons Inc.",

number = "20",

}

TY - JOUR

T1 - A scanning peptide array approach uncovers association sites within the JNK/βarrestin signalling complex

AU - Li, Xiang

AU - MacLeod, Ruth

AU - Dunlop, Allan J.

AU - Edwards, Helen V.

AU - Advant, Noopur

AU - Gibson, L. C D

AU - Devine, Nicola M.

AU - Brown, Kim M.

AU - Adams, David R.

AU - Houslay, Miles D.

AU - Baillie, George S.

PY - 2009/10/20

Y1 - 2009/10/20

N2 - ßarrestins are molecular scaffolds that can bring together three-component mitogen-activated protein kinase signalling modules to promote signal compartmentalisation. We use peptide array technology to define novel interfaces between components within the c-Jun N-terminal kinase (JNK)/ßarrestin signalling complex. We show that ßarrestin 1 and ßarrestin 2 associate with JNK3 via the kinase N-terminal domain in a region that, surprisingly, does not harbour a known 'common docking' motif. In the N-domain and C-terminus of ßarrestin 1 and ßarrestin 2 we identify two novel apoptosis signal-regulating kinase 1 binding sites and in the N-domain of the ßarrestin 1 and ßarrestin 2 we identify a novel MKK4 docking site. Structured summary: MINT-7263196, MINT-7263175: Arrestin beta-2 (uniprotkb:P32121) binds (MI:0407) to ASK1 (uniprotkb:Q99683) by peptide array (MI:0081). MINT-7263136: JNK3 (uniprotkb:P53779) binds (MI:0407) to Arrestin beta-1 (uniprotkb:P49407) by peptide array (MI:0081). MINT-7263161: JNK3 (uniprotkb:P53779) binds (MI:0407) to Arrestin beta-2 (uniprotkb:P32121) by peptide array (MI:0081). MINT-7263304: Arrestin beta-1 (uniprotkb:P49407) physically interacts (MI:0915) with ASK1 (uniprotkb:Q99683) by anti tag coimmunoprecipitation (MI:0007). MINT-7263286: Arrestin beta-2 (uniprotkb:P32121) binds (MI:0407) to MKK4 (uniprotkb:P45985) by peptide array (MI:0081). MINT-7263231, MINT-7263254: Arrestin beta-1 (uniprotkb:P49407) binds (MI:0407) to ASK1 (uniprotkb:Q99683) by peptide array (MI:0081). MINT-7263269: Arrestin beta-1 (uniprotkb:P49407) binds (MI:0407) to MKK4 (uniprotkb:P45985) by peptide array (MI:0081). © 2009 Federation of European Biochemical Societies.

AB - ßarrestins are molecular scaffolds that can bring together three-component mitogen-activated protein kinase signalling modules to promote signal compartmentalisation. We use peptide array technology to define novel interfaces between components within the c-Jun N-terminal kinase (JNK)/ßarrestin signalling complex. We show that ßarrestin 1 and ßarrestin 2 associate with JNK3 via the kinase N-terminal domain in a region that, surprisingly, does not harbour a known 'common docking' motif. In the N-domain and C-terminus of ßarrestin 1 and ßarrestin 2 we identify two novel apoptosis signal-regulating kinase 1 binding sites and in the N-domain of the ßarrestin 1 and ßarrestin 2 we identify a novel MKK4 docking site. Structured summary: MINT-7263196, MINT-7263175: Arrestin beta-2 (uniprotkb:P32121) binds (MI:0407) to ASK1 (uniprotkb:Q99683) by peptide array (MI:0081). MINT-7263136: JNK3 (uniprotkb:P53779) binds (MI:0407) to Arrestin beta-1 (uniprotkb:P49407) by peptide array (MI:0081). MINT-7263161: JNK3 (uniprotkb:P53779) binds (MI:0407) to Arrestin beta-2 (uniprotkb:P32121) by peptide array (MI:0081). MINT-7263304: Arrestin beta-1 (uniprotkb:P49407) physically interacts (MI:0915) with ASK1 (uniprotkb:Q99683) by anti tag coimmunoprecipitation (MI:0007). MINT-7263286: Arrestin beta-2 (uniprotkb:P32121) binds (MI:0407) to MKK4 (uniprotkb:P45985) by peptide array (MI:0081). MINT-7263231, MINT-7263254: Arrestin beta-1 (uniprotkb:P49407) binds (MI:0407) to ASK1 (uniprotkb:Q99683) by peptide array (MI:0081). MINT-7263269: Arrestin beta-1 (uniprotkb:P49407) binds (MI:0407) to MKK4 (uniprotkb:P45985) by peptide array (MI:0081). © 2009 Federation of European Biochemical Societies.

KW - Arrestin

KW - ASK1

KW - JNK

KW - MKK4

KW - Peptide array

KW - Scaffold

UR - https://www.scopus.com/pages/publications/70349878646

U2 - 10.1016/j.febslet.2009.09.035

DO - 10.1016/j.febslet.2009.09.035

M3 - Article

C2 - 19782076

SN - 0014-5793

VL - 583

SP - 3310

EP - 3316

JO - FEBS Letters

JF - FEBS Letters

IS - 20

ER -

A scanning peptide array approach uncovers association sites within the JNK/βarrestin signalling complex

Abstract

Keywords

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