A rapid and simple method to isolate pure alpha-cellulose

O. Brendel, P.P.M. Iannetta, D. Stewart

    Research output: Contribution to journalArticle

    181 Citations (Scopus)

    Abstract

    A protocol is described to isolate small quantities of highly purified cellulose for isotopic analysis of 10–100 mg samples of secondary (Pinus sylvestris L.) and primary (Rubus idaeus L.) plant cell wall material. Elemental analysis of 350 cellulose samples isolated from pine wood samples estimated the relative carbon content to be ca. 43.7% ± 1.2%. This value indicates that the cellulose quality is high and that the protocol is highly reproducible. High-performance anion exchange chromatography with pulsed amperometric detection of hydrolysis products quantified the purity of the cellulose as ca. 99% for wood cellulose and primary cell wall cellulose. DRIFT spectroscopy corroborated this purity and found no evidence of cellulose degradation. Carbon isotopic composition of the purified cellulose using mass spectrometry was measured with an accuracy of 0.11‰ (standard deviation). The method is rapid (56 samples may be routinely processed within 8 h) and requires only standard laboratory equipment and chemicals. Copyright © 2000 John Wiley & Sons, Ltd.
    Original languageEnglish
    Pages (from-to)7-10
    Number of pages4
    JournalPhytochemical Analysis
    Volume11
    Issue number1
    DOIs
    Publication statusPublished - 2000

    Keywords

    • cellulose
    • delignification
    • purification
    • pine wood
    • raspberry fruit
    • stable isotopes

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