A PORTABLE INTEGRATED WORKFLOW TO IDENTIFY SEPSIS CAUSING PATHOGENS USING CELL-FREE CIRCULATING MICROBIAL DNA

Ana Martinez-Lopez; Linda Marriott; Antonio Liga; Amanda Warr; Nicholas R. Leslie; Maïwenn Kersaudy-Kerhoas

A PORTABLE INTEGRATED WORKFLOW TO IDENTIFY SEPSIS CAUSING PATHOGENS USING CELL-FREE CIRCULATING MICROBIAL DNA

Ana Martinez-Lopez, Linda Marriott, Antonio Liga, Amanda Warr, Nicholas R. Leslie, Maïwenn Kersaudy-Kerhoas^*

^*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

Being able to rapidly identify causative agents will help to make rational medical decisions in the clinical management of sepsis. Microbial cell-free DNA released from lysed pathogens in the infected human circulation has been shown to have high sensitivity for detection of pathogens. Here we present the combination of a novel portable platform for cfDNA extraction and real-time sequencing protocol and we demonstrate the identification of circulating microbial sequences in under 6h.

Original language	English
Title of host publication	25th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2021)
Place of Publication	9781713855736
Publisher	Chemical and Biological Microsystems Society
Pages	693-694
Number of pages	2
Volume	1
ISBN (Electronic)	9781733419031
Publication status	Published - Oct 2021
Event	25th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2021 - Palm Springs, Virtual, United States Duration: 10 Oct 2021 → 14 Oct 2021 https://www.microtasconferences.org/microtas2021/

Conference

Conference	25th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2021
Abbreviated title	MicroTAS 2021
Country/Territory	United States
City	Palm Springs, Virtual
Period	10/10/21 → 14/10/21
Internet address	https://www.microtasconferences.org/microtas2021/

Keywords

Cell-free Circulating microbial DNA
Sample Preparation
Sepsis
Sequencing

ASJC Scopus subject areas

Bioengineering
Chemical Engineering (miscellaneous)

Access to Document

https://www.proceedings.com/64786.html

Cite this

Martinez-Lopez, A., Marriott, L., Liga, A., Warr, A., Leslie, N. R., & Kersaudy-Kerhoas, M. (2021). A PORTABLE INTEGRATED WORKFLOW TO IDENTIFY SEPSIS CAUSING PATHOGENS USING CELL-FREE CIRCULATING MICROBIAL DNA. In 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2021) (Vol. 1, pp. 693-694). Chemical and Biological Microsystems Society. https://www.proceedings.com/64786.html

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title = "A PORTABLE INTEGRATED WORKFLOW TO IDENTIFY SEPSIS CAUSING PATHOGENS USING CELL-FREE CIRCULATING MICROBIAL DNA",

abstract = "Being able to rapidly identify causative agents will help to make rational medical decisions in the clinical management of sepsis. Microbial cell-free DNA released from lysed pathogens in the infected human circulation has been shown to have high sensitivity for detection of pathogens. Here we present the combination of a novel portable platform for cfDNA extraction and real-time sequencing protocol and we demonstrate the identification of circulating microbial sequences in under 6h.",

keywords = "Cell-free Circulating microbial DNA, Sample Preparation, Sepsis, Sequencing",

author = "Ana Martinez-Lopez and Linda Marriott and Antonio Liga and Amanda Warr and Leslie, {Nicholas R.} and Ma{\"i}wenn Kersaudy-Kerhoas",

note = "Publisher Copyright: {\textcopyright} 2021 MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences. All rights reserved.; 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2021, MicroTAS 2021 ; Conference date: 10-10-2021 Through 14-10-2021",

year = "2021",

month = oct,

language = "English",

volume = "1",

pages = "693--694",

booktitle = "25th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2021)",

publisher = "Chemical and Biological Microsystems Society",

url = "https://www.microtasconferences.org/microtas2021/",

}

Martinez-Lopez, A, Marriott, L, Liga, A, Warr, A, Leslie, NR & Kersaudy-Kerhoas, M 2021, A PORTABLE INTEGRATED WORKFLOW TO IDENTIFY SEPSIS CAUSING PATHOGENS USING CELL-FREE CIRCULATING MICROBIAL DNA. in 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2021). vol. 1, Chemical and Biological Microsystems Society, 9781713855736, pp. 693-694, 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2021, Palm Springs, Virtual, California, United States, 10/10/21. <https://www.proceedings.com/64786.html>

A PORTABLE INTEGRATED WORKFLOW TO IDENTIFY SEPSIS CAUSING PATHOGENS USING CELL-FREE CIRCULATING MICROBIAL DNA. / Martinez-Lopez, Ana; Marriott, Linda; Liga, Antonio et al.
25th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2021). Vol. 1 9781713855736: Chemical and Biological Microsystems Society, 2021. p. 693-694.

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

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T1 - A PORTABLE INTEGRATED WORKFLOW TO IDENTIFY SEPSIS CAUSING PATHOGENS USING CELL-FREE CIRCULATING MICROBIAL DNA

AU - Martinez-Lopez, Ana

AU - Marriott, Linda

AU - Liga, Antonio

AU - Warr, Amanda

AU - Leslie, Nicholas R.

AU - Kersaudy-Kerhoas, Maïwenn

PY - 2021/10

Y1 - 2021/10

N2 - Being able to rapidly identify causative agents will help to make rational medical decisions in the clinical management of sepsis. Microbial cell-free DNA released from lysed pathogens in the infected human circulation has been shown to have high sensitivity for detection of pathogens. Here we present the combination of a novel portable platform for cfDNA extraction and real-time sequencing protocol and we demonstrate the identification of circulating microbial sequences in under 6h.

AB - Being able to rapidly identify causative agents will help to make rational medical decisions in the clinical management of sepsis. Microbial cell-free DNA released from lysed pathogens in the infected human circulation has been shown to have high sensitivity for detection of pathogens. Here we present the combination of a novel portable platform for cfDNA extraction and real-time sequencing protocol and we demonstrate the identification of circulating microbial sequences in under 6h.

KW - Cell-free Circulating microbial DNA

KW - Sample Preparation

KW - Sepsis

KW - Sequencing

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M3 - Conference contribution

AN - SCOPUS:85136979362

VL - 1

SP - 693

EP - 694

BT - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2021)

PB - Chemical and Biological Microsystems Society

CY - 9781713855736

T2 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2021

Y2 - 10 October 2021 through 14 October 2021

ER -

Martinez-Lopez A, Marriott L, Liga A, Warr A, Leslie NR , Kersaudy-Kerhoas M. A PORTABLE INTEGRATED WORKFLOW TO IDENTIFY SEPSIS CAUSING PATHOGENS USING CELL-FREE CIRCULATING MICROBIAL DNA. In 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2021). Vol. 1. 9781713855736: Chemical and Biological Microsystems Society. 2021. p. 693-694

A PORTABLE INTEGRATED WORKFLOW TO IDENTIFY SEPSIS CAUSING PATHOGENS USING CELL-FREE CIRCULATING MICROBIAL DNA

Abstract

Conference

Keywords

ASJC Scopus subject areas

Access to Document

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