A Novel Multi-pad Paper Plate (MP3) Based Assays for Rapid Animal Disease Diagnostics: Proceedings of the 30th anniversary Eurosensors Conference – Eurosensors 2016, 4-7. Sepember 2016, Budapest, Hungary

Valentina Busin, Stewart T. G. Burgess, Wenmaio Shu

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Abstract This study describes a novel fabrication method of a multi-pad paper plate (MP3) for transfer and optimisation of enzyme-linked immunosorbent assays (ELISA) on paper. The plate was fabricated using a combination of laser cutting and lamination for rapid and low-cost production of a multi-pad paper plate compatible with a standard 96 well microplate format. The plate was used to transfer a sandwich ELISA assay for detection of the acute phase protein bovine haptoglobin, allowing assay optimisation in the new format. The optimised protocol was also applied to detection of haptoglobin in bovine serum, demonstrating significant correlation with the traditional lab-based ELISA.
Original languageEnglish
Pages (from-to)1418-1421
Number of pages4
JournalProcedia Engineering
Volume168
DOIs
Publication statusPublished - 2016

Keywords

  • P-ELISA
  • microfluidics
  • veterinary medicine
  • haptoglobin
  • point-of-care

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