A Novel Multi-pad Paper Plate (MP3) Based Assays for Rapid Animal Disease Diagnostics: Proceedings of the 30th anniversary Eurosensors Conference – Eurosensors 2016, 4-7. Sepember 2016, Budapest, Hungary

Valentina Busin; Stewart T. G. Burgess; Wenmaio Shu

doi:10.1016/j.proeng.2016.11.402

A Novel Multi-pad Paper Plate (MP3) Based Assays for Rapid Animal Disease Diagnostics: Proceedings of the 30th anniversary Eurosensors Conference – Eurosensors 2016, 4-7. Sepember 2016, Budapest, Hungary

Valentina Busin, Stewart T. G. Burgess, Wenmaio Shu

School of Engineering & Physical Sciences

Research output: Contribution to journal › Article › peer-review

6 Citations (Scopus)

Abstract

Abstract This study describes a novel fabrication method of a multi-pad paper plate (MP3) for transfer and optimisation of enzyme-linked immunosorbent assays (ELISA) on paper. The plate was fabricated using a combination of laser cutting and lamination for rapid and low-cost production of a multi-pad paper plate compatible with a standard 96 well microplate format. The plate was used to transfer a sandwich ELISA assay for detection of the acute phase protein bovine haptoglobin, allowing assay optimisation in the new format. The optimised protocol was also applied to detection of haptoglobin in bovine serum, demonstrating significant correlation with the traditional lab-based ELISA.

Original language	English
Pages (from-to)	1418-1421
Number of pages	4
Journal	Procedia Engineering
Volume	168
DOIs	https://doi.org/10.1016/j.proeng.2016.11.402
Publication status	Published - 2016

Keywords

P-ELISA
microfluidics
veterinary medicine
haptoglobin
point-of-care

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10.1016/j.proeng.2016.11.402Licence: CC BY-NC-ND

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title = "A Novel Multi-pad Paper Plate (MP3) Based Assays for Rapid Animal Disease Diagnostics: Proceedings of the 30th anniversary Eurosensors Conference – Eurosensors 2016, 4-7. Sepember 2016, Budapest, Hungary",

abstract = "Abstract This study describes a novel fabrication method of a multi-pad paper plate (MP3) for transfer and optimisation of enzyme-linked immunosorbent assays (ELISA) on paper. The plate was fabricated using a combination of laser cutting and lamination for rapid and low-cost production of a multi-pad paper plate compatible with a standard 96 well microplate format. The plate was used to transfer a sandwich ELISA assay for detection of the acute phase protein bovine haptoglobin, allowing assay optimisation in the new format. The optimised protocol was also applied to detection of haptoglobin in bovine serum, demonstrating significant correlation with the traditional lab-based ELISA.",

keywords = "P-ELISA, microfluidics, veterinary medicine, haptoglobin, point-of-care",

author = "Valentina Busin and Burgess, {Stewart T. G.} and Wenmaio Shu",

year = "2016",

doi = "10.1016/j.proeng.2016.11.402",

language = "English",

volume = "168",

pages = "1418--1421",

journal = "Procedia Engineering",

issn = "1877-7058",

publisher = "Elsevier",

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A Novel Multi-pad Paper Plate (MP3) Based Assays for Rapid Animal Disease Diagnostics: Proceedings of the 30th anniversary Eurosensors Conference – Eurosensors 2016, 4-7. Sepember 2016, Budapest, Hungary. / Busin, Valentina; Burgess, Stewart T. G.; Shu, Wenmaio.
In: Procedia Engineering, Vol. 168, 2016, p. 1418-1421.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

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AU - Busin, Valentina

AU - Burgess, Stewart T. G.

AU - Shu, Wenmaio

PY - 2016

Y1 - 2016

N2 - Abstract This study describes a novel fabrication method of a multi-pad paper plate (MP3) for transfer and optimisation of enzyme-linked immunosorbent assays (ELISA) on paper. The plate was fabricated using a combination of laser cutting and lamination for rapid and low-cost production of a multi-pad paper plate compatible with a standard 96 well microplate format. The plate was used to transfer a sandwich ELISA assay for detection of the acute phase protein bovine haptoglobin, allowing assay optimisation in the new format. The optimised protocol was also applied to detection of haptoglobin in bovine serum, demonstrating significant correlation with the traditional lab-based ELISA.

AB - Abstract This study describes a novel fabrication method of a multi-pad paper plate (MP3) for transfer and optimisation of enzyme-linked immunosorbent assays (ELISA) on paper. The plate was fabricated using a combination of laser cutting and lamination for rapid and low-cost production of a multi-pad paper plate compatible with a standard 96 well microplate format. The plate was used to transfer a sandwich ELISA assay for detection of the acute phase protein bovine haptoglobin, allowing assay optimisation in the new format. The optimised protocol was also applied to detection of haptoglobin in bovine serum, demonstrating significant correlation with the traditional lab-based ELISA.

KW - P-ELISA

KW - microfluidics

KW - veterinary medicine

KW - haptoglobin

KW - point-of-care

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