A novel method for subcellular fractionation of Saccharomyces cerevisiae

Kevin J. Blackwell; Jane S. White; John M. Tobin

doi:10.1023/A:1008954002576

A novel method for subcellular fractionation of Saccharomyces cerevisiae

Kevin J. Blackwell, Jane S. White^*, John M. Tobin

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

2 Citations (Scopus)

Abstract

A novel technique for differential extraction of subcellular ion pools from Saccharomyces cerevisiae was developed. Glyceraldehyde-3-phosphate dehydrogenase and carboxypeptidase Y were chosen as biochemical markers for the cytoplasmic and vacuolar fractions, respectively. Approximately 70% of cytosolic and vacuolar markers were detected in the relevant fractions. Sr²⁺, Mn²⁺ and Cd²⁺ were localised using this method.

Original language	English
Pages (from-to)	583-587
Number of pages	5
Journal	Biotechnology Techniques
Volume	13
Issue number	9
DOIs	https://doi.org/10.1023/A:1008954002576
Publication status	Published - Sept 1999

Keywords

Biochemical markers
Cytosol
Metal localisation
Subcellular fractionation
Vacuole

ASJC Scopus subject areas

Biochemistry
Applied Microbiology and Biotechnology

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10.1023/A:1008954002576

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title = "A novel method for subcellular fractionation of Saccharomyces cerevisiae",

abstract = "A novel technique for differential extraction of subcellular ion pools from Saccharomyces cerevisiae was developed. Glyceraldehyde-3-phosphate dehydrogenase and carboxypeptidase Y were chosen as biochemical markers for the cytoplasmic and vacuolar fractions, respectively. Approximately 70\% of cytosolic and vacuolar markers were detected in the relevant fractions. Sr2+, Mn2+ and Cd2+ were localised using this method.",

keywords = "Biochemical markers, Cytosol, Metal localisation, Subcellular fractionation, Vacuole",

author = "Blackwell, \{Kevin J.\} and White, \{Jane S.\} and Tobin, \{John M.\}",

year = "1999",

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doi = "10.1023/A:1008954002576",

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volume = "13",

pages = "583--587",

journal = "Biotechnology Techniques",

issn = "0951-208X",

publisher = "Kluwer Academic Publishers",

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TY - JOUR

T1 - A novel method for subcellular fractionation of Saccharomyces cerevisiae

AU - Blackwell, Kevin J.

AU - White, Jane S.

AU - Tobin, John M.

PY - 1999/9

Y1 - 1999/9

N2 - A novel technique for differential extraction of subcellular ion pools from Saccharomyces cerevisiae was developed. Glyceraldehyde-3-phosphate dehydrogenase and carboxypeptidase Y were chosen as biochemical markers for the cytoplasmic and vacuolar fractions, respectively. Approximately 70% of cytosolic and vacuolar markers were detected in the relevant fractions. Sr2+, Mn2+ and Cd2+ were localised using this method.

AB - A novel technique for differential extraction of subcellular ion pools from Saccharomyces cerevisiae was developed. Glyceraldehyde-3-phosphate dehydrogenase and carboxypeptidase Y were chosen as biochemical markers for the cytoplasmic and vacuolar fractions, respectively. Approximately 70% of cytosolic and vacuolar markers were detected in the relevant fractions. Sr2+, Mn2+ and Cd2+ were localised using this method.

KW - Biochemical markers

KW - Cytosol

KW - Metal localisation

KW - Subcellular fractionation

KW - Vacuole

UR - https://www.scopus.com/pages/publications/0032874128

U2 - 10.1023/A:1008954002576

DO - 10.1023/A:1008954002576

M3 - Article

AN - SCOPUS:0032874128

SN - 0951-208X

VL - 13

SP - 583

EP - 587

JO - Biotechnology Techniques

JF - Biotechnology Techniques

IS - 9

ER -

A novel method for subcellular fractionation of Saccharomyces cerevisiae

Abstract

Keywords

ASJC Scopus subject areas

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