A novel technique for differential extraction of subcellular ion pools from Saccharomyces cerevisiae was developed. Glyceraldehyde-3-phosphate dehydrogenase and carboxypeptidase Y were chosen as biochemical markers for the cytoplasmic and vacuolar fractions, respectively. Approximately 70% of cytosolic and vacuolar markers were detected in the relevant fractions. Sr2+, Mn2+ and Cd2+ were localised using this method.
- Biochemical markers
- Metal localisation
- Subcellular fractionation
ASJC Scopus subject areas
- Applied Microbiology and Biotechnology