A novel method for subcellular fractionation of Saccharomyces cerevisiae

Kevin J. Blackwell, Jane S. White, John M. Tobin

Research output: Contribution to journalArticle

Abstract

A novel technique for differential extraction of subcellular ion pools from Saccharomyces cerevisiae was developed. Glyceraldehyde-3-phosphate dehydrogenase and carboxypeptidase Y were chosen as biochemical markers for the cytoplasmic and vacuolar fractions, respectively. Approximately 70% of cytosolic and vacuolar markers were detected in the relevant fractions. Sr2+, Mn2+ and Cd2+ were localised using this method.

Original languageEnglish
Pages (from-to)583-587
Number of pages5
JournalBiotechnology Techniques
Volume13
Issue number9
DOIs
Publication statusPublished - Sep 1999

Keywords

  • Biochemical markers
  • Cytosol
  • Metal localisation
  • Subcellular fractionation
  • Vacuole

ASJC Scopus subject areas

  • Biochemistry
  • Applied Microbiology and Biotechnology

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