A novel leptin signalling pathway via PTEN inhibition in hypothalamic cell lines and pancreatic beta-cells

Ke Ning, Lisa C Miller, Hilary A Laidlaw, Laura A Burgess, Nevin M Perera, C Peter Downes, Nick R Leslie, Michael L J Ashford

Research output: Contribution to journalArticlepeer-review

92 Citations (Scopus)


In obesity and diabetes, the ability of hypothalamic neurons to sense and transduce changes in leptin and insulin levels is compromised. The effects of both hormones require intracellular signalling via the PI3-kinase pathway, which is inhibited by the phosphatase PTEN. We show that leptin-stimulated F-actin depolymerization in mouse hypothalamic cells is inhibited by PTEN, a process involving independent effects of both its lipid and protein phosphatase activities. Potentially mediating this F-actin depolymerization, leptin, but not insulin, stimulated the phosphorylation of PTEN in a CK2 dependent manner, and inhibited its phosphatase activity. Similarly, hyperpolarization of mouse pancreatic beta-cells by leptin also requires coincident PtdIns(3,4,5)P3 generation and actin depolymerization, and could be inhibited by mechanisms requiring both the lipid and protein phosphatase activities of PTEN. These results demonstrate a critical role for PTEN in leptin signalling and indicate a mechanism by which leptin and insulin can produce PI3K dependent differential cellular outputs.
Original languageEnglish
Pages (from-to)2377-2387
Number of pages11
JournalEMBO Journal
Issue number11
Publication statusPublished - 7 Jun 2006


  • Actins
  • Animals
  • Cells, Cultured
  • Hypothalamus
  • Insulin-Secreting Cells
  • Leptin
  • Mice
  • PTEN Phosphohydrolase
  • Patch-Clamp Techniques
  • Phosphatidylinositol 3-Kinases
  • Phosphatidylinositol Phosphates
  • Phosphorylation
  • Potassium Channels
  • RNA, Small Interfering
  • Receptors, Cell Surface
  • Receptors, Leptin
  • Signal Transduction


Dive into the research topics of 'A novel leptin signalling pathway via PTEN inhibition in hypothalamic cell lines and pancreatic beta-cells'. Together they form a unique fingerprint.

Cite this