A microfluidic finger-actuated blood lysate preparation device enabled by rapid acoustofluidic mixing

Md Ehtashamul Haque, Alvaro J. Conde, William N. MacPherson, Stephen R. Knight, Richard M. Carter, Maïwenn Kersaudy-Kerhoas

Research output: Contribution to journalArticlepeer-review

5 Citations (Scopus)
71 Downloads (Pure)

Abstract

For many blood-based diagnostic tests, including prophylactic drug analysis and malaria assays, red blood cells must be lysed effectively prior to their use in an analytical workflow. We report on a finger-actuated blood lysate preparation device, which utilises a previously reported acoustofluidic micromixer module. The integrated device includes a range of innovations from a sample interface, to the integration of blisters on a laser engraved surface and a large volume (130 μL) one-stroke manual pump which could be useful in other low-cost microfluidic-based point-of-care devices. The adaptability of the acoustic mixer is demonstrated on highly viscous fluids, including whole blood, with up to 65% percent volume fraction of red blood cells. Used in conjunction with a lysis buffer, the micromixer unit is also shown to lyse a finger-prick (approximately 20 μL) blood sample in 30 seconds and benchmarked across ten donor samples. Finally, we demonstrate the ease of use of the fully integrated device. Cheap, modular, but reliable, finger-actuated microfluidic functions could open up opportunities for the development of diagnostics with minimal resources.

Original languageEnglish
Pages (from-to)62-71
Number of pages10
JournalLab on a Chip
Volume23
Issue number1
Early online date30 Nov 2022
DOIs
Publication statusPublished - 7 Jan 2023

Keywords

  • Microfluidic Analytical Techniques
  • Erythrocytes
  • Microfluidics
  • Fingers
  • Lab-On-A-Chip Devices

ASJC Scopus subject areas

  • Bioengineering
  • General Chemistry
  • Biochemistry
  • Biomedical Engineering

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