A micro-rheological and rheological study of biopolymers solutions: Hyaluronic acid

Andrea Dodero, Rhodri Williams, Simona Gagliardi, Silvia Vicini, Marina Alloisio, Maila Castellano

Research output: Contribution to journalArticle

Abstract

Hyaluronic acid (HA) solutions represent an important class of biomedical products, mostly used as viscosupplements in orthopaedics and as fillers in the cosmetic industry. The focus of the present work is the hydrodynamic, micro-rheological and rheological characterization of HA in physiological saline. Standard viscoelastic characterization techniques were coupled with micro-rheological measurements, i.e. by measuring the passive motions of particles embedded in the samples via Dynamic Light Scattering (DLS), effectively extending the accessible frequency range typical of standard rheometers. The influence of molecular weight and polymer concentration on the storage modulus (G’), loss modulus (G”) and complex viscosity (η*) of HA saline solutions was investigated. A brief comparison with theoretical models was made showing such concentrated solutions to be of a semi-flexible nature. In addition, the entanglement concentration ν, the critical molecular weight , and the mesh size ξ of the physical network created by the entangled polymer chains were calculated.
LanguageEnglish
Pages349-355
Number of pages7
JournalCarbohydrate Polymers
Volume203
Early online date28 Sep 2018
DOIs
StatePublished - 1 Jan 2019

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Biopolymers
Hyaluronic Acid
Viscosupplements
Polymers
Molecular weight
Cosmetics
Rheometers
Orthopedics
Dynamic light scattering
Sodium Chloride
Fillers
Hydrodynamics
Elastic moduli
Viscosity
Industry

Cite this

Dodero, A., Williams, R., Gagliardi, S., Vicini, S., Alloisio, M., & Castellano, M. (2019). A micro-rheological and rheological study of biopolymers solutions: Hyaluronic acid. Carbohydrate Polymers, 203, 349-355. DOI: 10.1016/j.carbpol.2018.09.072
Dodero, Andrea ; Williams, Rhodri ; Gagliardi, Simona ; Vicini, Silvia ; Alloisio, Marina ; Castellano, Maila. / A micro-rheological and rheological study of biopolymers solutions: Hyaluronic acid. In: Carbohydrate Polymers. 2019 ; Vol. 203. pp. 349-355
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Dodero, A, Williams, R, Gagliardi, S, Vicini, S, Alloisio, M & Castellano, M 2019, 'A micro-rheological and rheological study of biopolymers solutions: Hyaluronic acid' Carbohydrate Polymers, vol. 203, pp. 349-355. DOI: 10.1016/j.carbpol.2018.09.072

A micro-rheological and rheological study of biopolymers solutions: Hyaluronic acid. / Dodero, Andrea; Williams, Rhodri; Gagliardi, Simona; Vicini, Silvia; Alloisio, Marina; Castellano, Maila.

In: Carbohydrate Polymers, Vol. 203, 01.01.2019, p. 349-355.

Research output: Contribution to journalArticle

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AB - Hyaluronic acid (HA) solutions represent an important class of biomedical products, mostly used as viscosupplements in orthopaedics and as fillers in the cosmetic industry. The focus of the present work is the hydrodynamic, micro-rheological and rheological characterization of HA in physiological saline. Standard viscoelastic characterization techniques were coupled with micro-rheological measurements, i.e. by measuring the passive motions of particles embedded in the samples via Dynamic Light Scattering (DLS), effectively extending the accessible frequency range typical of standard rheometers. The influence of molecular weight and polymer concentration on the storage modulus (G’), loss modulus (G”) and complex viscosity (η*) of HA saline solutions was investigated. A brief comparison with theoretical models was made showing such concentrated solutions to be of a semi-flexible nature. In addition, the entanglement concentration ν, the critical molecular weight , and the mesh size ξ of the physical network created by the entangled polymer chains were calculated.

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Dodero A, Williams R, Gagliardi S, Vicini S, Alloisio M, Castellano M. A micro-rheological and rheological study of biopolymers solutions: Hyaluronic acid. Carbohydrate Polymers. 2019 Jan 1;203:349-355. Available from, DOI: 10.1016/j.carbpol.2018.09.072