A hybrid discrete-continuum mathematical model of pattern prediction in the developing retinal vasculature

Steven Robert McDougall, Michael Greg Watson, A.H. Devlin, C.A. Mitchell, M.A.J. Chaplain

    Research output: Contribution to journalArticle

    Abstract

    Pathological angiogenesis has been extensively explored by the mathematical modelling community over the past few decades, specifically in the contexts of tumour-induced vascularisation and wound healing. However, there have been relatively few attempts to model angiogenesis associated with normal development, despite the availability of animal models with experimentally accessible and highly ordered vascular topologies: for example, growth and development of the vascular plexus layers in the murine retina. The current study aims to address this issue through the development of a hybrid discrete-continuum mathematical model of the developing retinal vasculature in neonatal mice that is closely coupled with an ongoing experimental programme. The model of the functional vasculature is informed by a range of morphological and molecular data obtained over a period of several days, from 6 days prior to birth to approximately 8 days after birth.
    The spatio-temporal formation of the superficial retinal vascular plexus (RVP) in wild-type mice occurs in a well-defined sequence. Prior to birth, astrocytes migrate from the optic nerve over the surface of the inner retina in response to a chemotactic gradient of PDGF-A, formed at an earlier stage by migrating retinal ganglion cells (RGCs). Astrocytes express a variety of chemotactic and haptotactic proteins, including VEGF and fibronectin (respectively), which subsequently induce endothelial cell sprouting and modulate growth of the RVP. The developing RVP is not an inert structure; however, the vascular bed adapts and remodels in response to a wide variety of metabolic and biomolecular stimuli. The main focus of this investigation is to understand how these interacting cellular, molecular, and metabolic cues regulate RVP growth and formation.
    In an earlier one-dimensional continuum model of astrocyte and endothelial migration, we showed that the measured frontal velocities of the two cell types could be accurately reproduced by means of a system of five coupled partial differential equations (Aubert et al. in Bull. Math. Biol. 73:2430–2451, 2011). However, this approach was unable to generate spatial information and structural detail for the entire retinal surface. Building upon this earlier work, a more realistic two-dimensional hybrid PDE-discrete model is derived here that tracks the migration of individual astrocytes and endothelial tip cells towards the outer retinal boundary. Blood perfusion is included throughout plexus development and the emergent retinal architectures adapt and remodel in response to various biological factors. The resulting in silico RVP structures are compared with whole-mounted retinal vasculatures at various stages of development, and the agreement is found to be excellent. Having successfully benchmarked the model against wild-type data, the effect of transgenic over-expression of various genes is predicted, based on the ocular-specific expression of VEGF-A during murine development. These results can be used to help inform future experimental investigations of signalling pathways in ocular conditions characterised by aberrant angiogenesis.
    Original languageEnglish
    Pages (from-to)2272-2314
    Number of pages43
    JournalBulletin of Mathematical Biology
    Volume74
    Issue number10
    DOIs
    Publication statusPublished - Oct 2012

    Fingerprint

    Retinal Vessels
    Theoretical Models
    Astrocytes
    Blood Vessels
    Parturition
    Vascular Endothelial Growth Factor A
    Retina
    Endothelial Cells
    Pathologic Neovascularization
    Retinal Ganglion Cells
    Biological Factors
    Optic Nerve
    Growth
    Growth and Development
    Fibronectins
    Computer Simulation
    Wound Healing
    Cues
    Animal Models
    Perfusion

    Cite this

    McDougall, Steven Robert ; Watson, Michael Greg ; Devlin, A.H. ; Mitchell, C.A. ; Chaplain, M.A.J. / A hybrid discrete-continuum mathematical model of pattern prediction in the developing retinal vasculature. In: Bulletin of Mathematical Biology. 2012 ; Vol. 74, No. 10. pp. 2272-2314.
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    A hybrid discrete-continuum mathematical model of pattern prediction in the developing retinal vasculature. / McDougall, Steven Robert; Watson, Michael Greg; Devlin, A.H.; Mitchell, C.A.; Chaplain, M.A.J.

    In: Bulletin of Mathematical Biology, Vol. 74, No. 10, 10.2012, p. 2272-2314.

    Research output: Contribution to journalArticle

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