Abstract
As a first step towards understanding the mechanism underlying the differential gene expression of the two variants of the rat proteinase-inhibitor a1-inhibitor 3 (a1-I3) corresponding genomic clones were isolated. The 100% similarity between the sequence of one genomic clone and that of the a1-I3 variant I cDNA strongly suggested that its 5'-sequence represented the upstream region of the corresponding gene. Several putative cis-regulatory elements were identified as well as a polypyrimidine tract located between the transcription start site of the a1-I3 variant I mRNA and the AUG codon. The polypyrimidine tract functions as a positive cis-element in a heterologous promoter. By electrophoretic mobility shift assays (EMSA) we have shown that a GST (glutathione S-transferase) fusion of the rat polypyrimidine tract binding protein (PTB) has a high affinity for the pyrimidine-rich sense strand but not for the complementary sequence of the 5'-untranslated region of the a1-I3 variant I gene.
Original language | English |
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Pages (from-to) | 1217-1223 |
Number of pages | 7 |
Journal | Biological Chemistry |
Volume | 380 |
Issue number | 10 |
DOIs | |
Publication status | Published - 1999 |
Keywords
- α 1 -inhibitor 3
- Acute phase protein
- Polypyrimidine-tract binding protein