A Fourier-transform infrared spectroscopic and microscopic study of the infection of potato tubers by Erwinia carotovora ssp. carotovora in aerobic and anaerobic conditions

D. Stewart, G.D Lyon, E.J.B. Tucker

    Research output: Contribution to journalArticle

    Abstract

    The infection or potato tubers (Solanum tuberosum) by Erwinia carotovora ssp carotovora in aerobic and anaerobic conditions was studied using chemical analysis and Fourier-transform infrared (FT-IR) microscopy and diffuse reflectance spectroscopy. Analysis or the uninfected and infected residues following sequential extraction with diethyl ether, water (40°C), 90% dimethyl sulphoxide (DMSO) and digestion with amyloglucosidase showed that the principal effect of infection, particularly in anaerobic conditions, is starch degradation. This was substantiated by gel filtration chromatography of the 90% DMSO extracts. Extensive lignification was not found to be intimately associated with either infective condition. FT-IR microscopical analysis of tissue sections showed that infection in both aerobic and anaerobic conditions caused starch and cell wall degradation, whilst extensive membrane degradation was only evident with anaerobic infection.
    Copyright © 1994 John Wiley & Sons, Ltd
    Original languageEnglish
    Pages (from-to)145-154
    Number of pages10
    JournalJournal of the Science of Food and Agriculture
    Volume66
    Issue number2
    DOIs
    Publication statusPublished - Oct 1994

    Keywords

    • potato
    • erwinia
    • DRIFT
    • FT-IR microscopy
    • cell wall
    • lignin
    • cross-linking

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