Abstract
The infection or potato tubers (Solanum tuberosum) by Erwinia carotovora ssp carotovora in aerobic and anaerobic conditions was studied using chemical analysis and Fourier-transform infrared (FT-IR) microscopy and diffuse reflectance spectroscopy. Analysis or the uninfected and infected residues following sequential extraction with diethyl ether, water (40°C), 90% dimethyl sulphoxide (DMSO) and digestion with amyloglucosidase showed that the principal effect of infection, particularly in anaerobic conditions, is starch degradation. This was substantiated by gel filtration chromatography of the 90% DMSO extracts. Extensive lignification was not found to be intimately associated with either infective condition. FT-IR microscopical analysis of tissue sections showed that infection in both aerobic and anaerobic conditions caused starch and cell wall degradation, whilst extensive membrane degradation was only evident with anaerobic infection.
Copyright © 1994 John Wiley & Sons, Ltd
Copyright © 1994 John Wiley & Sons, Ltd
| Original language | English |
|---|---|
| Pages (from-to) | 145-154 |
| Number of pages | 10 |
| Journal | Journal of the Science of Food and Agriculture |
| Volume | 66 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - Oct 1994 |
Keywords
- potato
- erwinia
- DRIFT
- FT-IR microscopy
- cell wall
- lignin
- cross-linking