TY - JOUR
T1 - A Flow Cytometry-based Method for the Screening of Nanomaterial-induced Reactive Oxygen Species Production in Leukocytes Subpopulations in Whole Blood
AU - Kermanizadeh, Ali
AU - Jantzen, Kim
AU - Brown, David McAllister
AU - Møller, Peter
AU - Loft, Steffen
PY - 2018/1/1
Y1 - 2018/1/1
N2 - To date, the use and translation of nanomedicine from the laboratory to the clinic has been relatively slow. Among other issues, one of the reasons for this tardiness is the lack of the availability of quick and reliable toxicity tools for the screening of nanomaterials (NMs). In this investigation, we apply a flow cytometry-based method for the detection of nanomaterial-induced oxidative stress by measurement of reactive oxygen species production in specific leukocyte subpopulations in human whole blood. The screening of a panel of relevant nanomedical-associated materials (liposomes, silica, iron oxide and functionalized single-walled carbon nanotubes) demonstrated that only the carbon nanotubes induced oxidative stress in human circulating leukocytes. In summary, we apply and corroborate a flow cytometry-based method for the simple and effective measurement of NM-induced oxidative stress in human blood subpopulations after realistic and relevant exposure scenarios which is extremely useful in future toxicological applications.
AB - To date, the use and translation of nanomedicine from the laboratory to the clinic has been relatively slow. Among other issues, one of the reasons for this tardiness is the lack of the availability of quick and reliable toxicity tools for the screening of nanomaterials (NMs). In this investigation, we apply a flow cytometry-based method for the detection of nanomaterial-induced oxidative stress by measurement of reactive oxygen species production in specific leukocyte subpopulations in human whole blood. The screening of a panel of relevant nanomedical-associated materials (liposomes, silica, iron oxide and functionalized single-walled carbon nanotubes) demonstrated that only the carbon nanotubes induced oxidative stress in human circulating leukocytes. In summary, we apply and corroborate a flow cytometry-based method for the simple and effective measurement of NM-induced oxidative stress in human blood subpopulations after realistic and relevant exposure scenarios which is extremely useful in future toxicological applications.
UR - http://www.scopus.com/inward/record.url?scp=85026840534&partnerID=8YFLogxK
U2 - 10.1111/bcpt.12845
DO - 10.1111/bcpt.12845
M3 - Article
C2 - 28691406
AN - SCOPUS:85026840534
SN - 1742-7835
VL - 122
SP - 149
EP - 156
JO - Basic and Clinical Pharmacology and Toxicology
JF - Basic and Clinical Pharmacology and Toxicology
IS - 1
ER -