A Flow Cytometry-based Method for the Screening of Nanomaterial-induced Reactive Oxygen Species Production in Leukocytes Subpopulations in Whole Blood

Ali Kermanizadeh, Kim Jantzen, David McAllister Brown, Peter Møller, Steffen Loft

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

To date, the use and translation of nanomedicine from the laboratory to the clinic has been relatively slow. Among other issues, one of the reasons for this tardiness is the lack of the availability of quick and reliable toxicity tools for the screening of nanomaterials (NMs). In this investigation, we apply a flow cytometry-based method for the detection of nanomaterial-induced oxidative stress by measurement of reactive oxygen species production in specific leukocyte subpopulations in human whole blood. The screening of a panel of relevant nanomedical-associated materials (liposomes, silica, iron oxide and functionalized single-walled carbon nanotubes) demonstrated that only the carbon nanotubes induced oxidative stress in human circulating leukocytes. In summary, we apply and corroborate a flow cytometry-based method for the simple and effective measurement of NM-induced oxidative stress in human blood subpopulations after realistic and relevant exposure scenarios which is extremely useful in future toxicological applications.

Original languageEnglish
Pages (from-to)149–156
Number of pages8
JournalBasic and Clinical Pharmacology and Toxicology
Volume122
Issue number1
Early online date6 Aug 2017
DOIs
Publication statusPublished - 1 Jan 2018

ASJC Scopus subject areas

  • Toxicology
  • Pharmacology

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