A Culture-Independent and Culture-Dependent Study of the Bacterial Community from the Bedrock Soil Interface

Karen Olsson-Francis, Carl P. Boardman, Victoria K. Pearson, Paul F. Schofield, Anna Oliver, Stephen Summers

Research output: Contribution to journalArticle

Abstract

In nutrient limited soils, minerals constitute a major reservoir of bio-essential elements. Consequently, the release of nutritive elements during weathering is crucial. Bacteria have been shown to enhance weathering rates; however, there has been limited work that has focused on the bacterial weathering of bedrock or parent rock, which are the major sources of minerals, in nutrient limiting soils. In this study, both a culture-independent and culture-dependent approach was used to study the bacterial community at the interface between basaltic bedrock and nutrient limiting soil in Cadiar Idris region of Snowdonia National Park, United Kingdom. High throughput sequencing method, Ion Torrent, was used to characterise the bacterial community, which generated over 250,000 sequences. Taxonomical assignment demonstrated that approximately 50% (125,000 sequences) of the community consisted of the orders Actinomycetales, Burkholderiales, Clostridales, Bacillales, Rhizobiales and Acidobacterium, with unclassified sequences representing 44% ± 1.46% (110,000 ± 3650). Bacteria belonging to the genera Serratia, Pseudomonas, Bacillus, Paenibacillus, Chromobacterium, Janthinobacterium, Burkholderia and Arthrobacter, were isolated from the sample site. All of the isolates were able to grow in a minimal growth medium, which contained glucose, ammonium chloride with basalt as the sole source of bio-essential elements. Seventy percent of the isolates significantly enhanced basalt dissolution (p <0.05). The rate of dissolution correlated to the production of oxalic acid and acidification of the growth medium. The findings of this work suggest that at the interface between bedrock and soil heterotrophic members of the bacterial community can enhance weathering, an essential part of biogeochemical cycling in nutrient limiting soil.
Original languageEnglish
Pages (from-to)842-857
Number of pages16
JournalAdvances in Microbiology
Volume5
DOIs
Publication statusPublished - Dec 2015

Fingerprint

bedrock
weathering
nutrient
soil
basalt
dissolution
torrent
bacterium
weathering rate
oxalic acid
mineral
soil nutrient
acidification
glucose
national park
ammonium
chloride
ion
rock

Keywords

  • Ba
  • Mineral Weathering
  • Soil Microbial Communities
  • basalt dissolution
  • mineral weathering
  • soil microbial communities

Cite this

Olsson-Francis, K., Boardman, C. P., Pearson, V. K., Schofield, P. F., Oliver, A., & Summers, S. (2015). A Culture-Independent and Culture-Dependent Study of the Bacterial Community from the Bedrock Soil Interface. Advances in Microbiology, 5, 842-857. https://doi.org/10.4236/aim.2015.513089
Olsson-Francis, Karen ; Boardman, Carl P. ; Pearson, Victoria K. ; Schofield, Paul F. ; Oliver, Anna ; Summers, Stephen. / A Culture-Independent and Culture-Dependent Study of the Bacterial Community from the Bedrock Soil Interface. In: Advances in Microbiology. 2015 ; Vol. 5. pp. 842-857.
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Olsson-Francis, K, Boardman, CP, Pearson, VK, Schofield, PF, Oliver, A & Summers, S 2015, 'A Culture-Independent and Culture-Dependent Study of the Bacterial Community from the Bedrock Soil Interface', Advances in Microbiology, vol. 5, pp. 842-857. https://doi.org/10.4236/aim.2015.513089

A Culture-Independent and Culture-Dependent Study of the Bacterial Community from the Bedrock Soil Interface. / Olsson-Francis, Karen; Boardman, Carl P.; Pearson, Victoria K.; Schofield, Paul F.; Oliver, Anna; Summers, Stephen.

In: Advances in Microbiology, Vol. 5, 12.2015, p. 842-857.

Research output: Contribution to journalArticle

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AU - Olsson-Francis, Karen

AU - Boardman, Carl P.

AU - Pearson, Victoria K.

AU - Schofield, Paul F.

AU - Oliver, Anna

AU - Summers, Stephen

PY - 2015/12

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AB - In nutrient limited soils, minerals constitute a major reservoir of bio-essential elements. Consequently, the release of nutritive elements during weathering is crucial. Bacteria have been shown to enhance weathering rates; however, there has been limited work that has focused on the bacterial weathering of bedrock or parent rock, which are the major sources of minerals, in nutrient limiting soils. In this study, both a culture-independent and culture-dependent approach was used to study the bacterial community at the interface between basaltic bedrock and nutrient limiting soil in Cadiar Idris region of Snowdonia National Park, United Kingdom. High throughput sequencing method, Ion Torrent, was used to characterise the bacterial community, which generated over 250,000 sequences. Taxonomical assignment demonstrated that approximately 50% (125,000 sequences) of the community consisted of the orders Actinomycetales, Burkholderiales, Clostridales, Bacillales, Rhizobiales and Acidobacterium, with unclassified sequences representing 44% ± 1.46% (110,000 ± 3650). Bacteria belonging to the genera Serratia, Pseudomonas, Bacillus, Paenibacillus, Chromobacterium, Janthinobacterium, Burkholderia and Arthrobacter, were isolated from the sample site. All of the isolates were able to grow in a minimal growth medium, which contained glucose, ammonium chloride with basalt as the sole source of bio-essential elements. Seventy percent of the isolates significantly enhanced basalt dissolution (p <0.05). The rate of dissolution correlated to the production of oxalic acid and acidification of the growth medium. The findings of this work suggest that at the interface between bedrock and soil heterotrophic members of the bacterial community can enhance weathering, an essential part of biogeochemical cycling in nutrient limiting soil.

KW - Ba

KW - Mineral Weathering

KW - Soil Microbial Communities

KW - basalt dissolution

KW - mineral weathering

KW - soil microbial communities

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DO - 10.4236/aim.2015.513089

M3 - Article

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EP - 857

JO - Advances in Microbiology

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