A comparison of singlet oxygen explicit dosimetry (SOED) and singlet oxygen luminescence dosimetry (SOLD) for photofrin-mediated photodynamic therapy

Michele M. Kim, Rozhin Penjweini, Nathan R. Gemmell, Israel Veilleux, Aongus McCarthy, Gerald Stuart Buller, Robert H. Hadfield, Brian C. Wilson, Timothy C. Zhu

Research output: Contribution to journalArticle

12 Citations (Scopus)
19 Downloads (Pure)

Abstract

Accurate photodynamic therapy (PDT) dosimetry is critical for the use of PDT in the treatment of malignant and nonmalignant localized diseases. A singlet oxygen explicit dosimetry (SOED) model has been developed for in vivo purposes. It involves the measurement of the key components in PDT-light fluence (rate), photosensitizer concentration, and ground-state oxygen concentration ([3O2])-to calculate the amount of reacted singlet oxygen ([1O2]rx), the main cytotoxic component in type II PDT. Experiments were performed in phantoms with the photosensitizer Photofrin and in solution using phosphorescence-based singlet oxygen luminescence dosimetry (SOLD) to validate the SOED model. Oxygen concentration and photosensitizer photobleaching versus time were measured during PDT, along with direct SOLD measurements of singlet oxygen and triplet state lifetime (τΔ and τt), for various photosensitizer concentrations to determine necessary photophysical parameters. SOLD-determined cumulative [1O2]rx was compared to SOED-calculated [1O2]rx for various photosensitizer concentrations to show a clear correlation between the two methods. This illustrates that explicit dosimetry can be used when phosphorescence-based dosimetry is not feasible. Using SOED modeling, we have also shown evidence that SOLD-measured [1O2]rx using a 523 nm pulsed laser can be used to correlate to singlet oxygen generated by a 630 nm laser during a clinical malignant pleural mesothelioma (MPM) PDT protocol by using a conversion formula.

Original languageEnglish
Article number109
JournalCancers
Volume8
Issue number12
DOIs
Publication statusPublished - 6 Dec 2016

Keywords

  • Oxygen
  • Photodynamic therapy
  • Photofrin
  • Singlet oxygen explicit dosimetry (SOED)
  • Singlet oxygen luminescence dosimetry (SOLD)

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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