A comparison of methods to assess cell mechanical properties

Pei Hsun Wu, Dikla Raz Ben Aroush, Atef Asnacios, Wei Chiang Chen, Maxim E. Dokukin, Bryant L. Doss, Pauline Durand-Smet, Andrew Ekpenyong, Jochen Guck, Nataliia V. Guz, Paul A. Janmey, Jerry S.H. Lee, Nicole M. Moore, Albrecht Ott, Yeh Chuin Poh, Robert Ros, Mathias Sander, Igor Sokolov, Jack R. Staunton, Ning Wang & 2 others Graeme Whyte, Denis Wirtz

Research output: Contribution to journalArticle

Abstract

The mechanical properties of cells influence their cellular and subcellular functions, including cell adhesion, migration, polarization, and differentiation, as well as organelle organization and trafficking inside the cytoplasm. Yet reported values of cell stiffness and viscosity vary substantially, which suggests differences in how the results of different methods are obtained or analyzed by different groups. To address this issue and illustrate the complementarity of certain approaches, here we present, analyze, and critically compare measurements obtained by means of some of the most widely used methods for cell mechanics: atomic force microscopy, magnetic twisting cytometry, particle-tracking microrheology, parallel-plate rheometry, cell monolayer rheology, and optical stretching. These measurements highlight how elastic and viscous moduli of MCF-7 breast cancer cells can vary 1,000-fold and 100-fold, respectively. We discuss the sources of these variations, including the level of applied mechanical stress, the rate of deformation, the geometry of the probe, the location probed in the cell, and the extracellular microenvironment.
LanguageEnglish
Pages491-498
Number of pages8
JournalNature Methods
Volume15
DOIs
StatePublished - 18 Jun 2018

Fingerprint

mechanical properties
cells
organelles
cytometry
cytoplasm
twisting
parallel plates
rheology
breast
stiffness
modulus of elasticity
adhesion
cancer
atomic force microscopy
viscosity
probes
polarization
geometry

Cite this

Wu, P. H., Aroush, D. R. B., Asnacios, A., Chen, W. C., Dokukin, M. E., Doss, B. L., ... Wirtz, D. (2018). A comparison of methods to assess cell mechanical properties. Nature Methods, 15, 491-498. DOI: 10.1038/s41592-018-0015-1
Wu, Pei Hsun ; Aroush, Dikla Raz Ben ; Asnacios, Atef ; Chen, Wei Chiang ; Dokukin, Maxim E. ; Doss, Bryant L. ; Durand-Smet, Pauline ; Ekpenyong, Andrew ; Guck, Jochen ; Guz, Nataliia V. ; Janmey, Paul A. ; Lee, Jerry S.H. ; Moore, Nicole M. ; Ott, Albrecht ; Poh, Yeh Chuin ; Ros, Robert ; Sander, Mathias ; Sokolov, Igor ; Staunton, Jack R. ; Wang, Ning ; Whyte, Graeme ; Wirtz, Denis. / A comparison of methods to assess cell mechanical properties. In: Nature Methods. 2018 ; Vol. 15. pp. 491-498
@article{d75b119c1e084fad96cb3926d8177d12,
title = "A comparison of methods to assess cell mechanical properties",
abstract = "The mechanical properties of cells influence their cellular and subcellular functions, including cell adhesion, migration, polarization, and differentiation, as well as organelle organization and trafficking inside the cytoplasm. Yet reported values of cell stiffness and viscosity vary substantially, which suggests differences in how the results of different methods are obtained or analyzed by different groups. To address this issue and illustrate the complementarity of certain approaches, here we present, analyze, and critically compare measurements obtained by means of some of the most widely used methods for cell mechanics: atomic force microscopy, magnetic twisting cytometry, particle-tracking microrheology, parallel-plate rheometry, cell monolayer rheology, and optical stretching. These measurements highlight how elastic and viscous moduli of MCF-7 breast cancer cells can vary 1,000-fold and 100-fold, respectively. We discuss the sources of these variations, including the level of applied mechanical stress, the rate of deformation, the geometry of the probe, the location probed in the cell, and the extracellular microenvironment.",
author = "Wu, {Pei Hsun} and Aroush, {Dikla Raz Ben} and Atef Asnacios and Chen, {Wei Chiang} and Dokukin, {Maxim E.} and Doss, {Bryant L.} and Pauline Durand-Smet and Andrew Ekpenyong and Jochen Guck and Guz, {Nataliia V.} and Janmey, {Paul A.} and Lee, {Jerry S.H.} and Moore, {Nicole M.} and Albrecht Ott and Poh, {Yeh Chuin} and Robert Ros and Mathias Sander and Igor Sokolov and Staunton, {Jack R.} and Ning Wang and Graeme Whyte and Denis Wirtz",
year = "2018",
month = "6",
day = "18",
doi = "10.1038/s41592-018-0015-1",
language = "English",
volume = "15",
pages = "491--498",
journal = "Nature Methods",
issn = "1548-7091",
publisher = "Nature Publishing Group",

}

Wu, PH, Aroush, DRB, Asnacios, A, Chen, WC, Dokukin, ME, Doss, BL, Durand-Smet, P, Ekpenyong, A, Guck, J, Guz, NV, Janmey, PA, Lee, JSH, Moore, NM, Ott, A, Poh, YC, Ros, R, Sander, M, Sokolov, I, Staunton, JR, Wang, N, Whyte, G & Wirtz, D 2018, 'A comparison of methods to assess cell mechanical properties' Nature Methods, vol. 15, pp. 491-498. DOI: 10.1038/s41592-018-0015-1

A comparison of methods to assess cell mechanical properties. / Wu, Pei Hsun; Aroush, Dikla Raz Ben; Asnacios, Atef; Chen, Wei Chiang; Dokukin, Maxim E.; Doss, Bryant L.; Durand-Smet, Pauline; Ekpenyong, Andrew; Guck, Jochen; Guz, Nataliia V.; Janmey, Paul A.; Lee, Jerry S.H.; Moore, Nicole M.; Ott, Albrecht; Poh, Yeh Chuin; Ros, Robert; Sander, Mathias; Sokolov, Igor; Staunton, Jack R.; Wang, Ning; Whyte, Graeme; Wirtz, Denis.

In: Nature Methods, Vol. 15, 18.06.2018, p. 491-498.

Research output: Contribution to journalArticle

TY - JOUR

T1 - A comparison of methods to assess cell mechanical properties

AU - Wu,Pei Hsun

AU - Aroush,Dikla Raz Ben

AU - Asnacios,Atef

AU - Chen,Wei Chiang

AU - Dokukin,Maxim E.

AU - Doss,Bryant L.

AU - Durand-Smet,Pauline

AU - Ekpenyong,Andrew

AU - Guck,Jochen

AU - Guz,Nataliia V.

AU - Janmey,Paul A.

AU - Lee,Jerry S.H.

AU - Moore,Nicole M.

AU - Ott,Albrecht

AU - Poh,Yeh Chuin

AU - Ros,Robert

AU - Sander,Mathias

AU - Sokolov,Igor

AU - Staunton,Jack R.

AU - Wang,Ning

AU - Whyte,Graeme

AU - Wirtz,Denis

PY - 2018/6/18

Y1 - 2018/6/18

N2 - The mechanical properties of cells influence their cellular and subcellular functions, including cell adhesion, migration, polarization, and differentiation, as well as organelle organization and trafficking inside the cytoplasm. Yet reported values of cell stiffness and viscosity vary substantially, which suggests differences in how the results of different methods are obtained or analyzed by different groups. To address this issue and illustrate the complementarity of certain approaches, here we present, analyze, and critically compare measurements obtained by means of some of the most widely used methods for cell mechanics: atomic force microscopy, magnetic twisting cytometry, particle-tracking microrheology, parallel-plate rheometry, cell monolayer rheology, and optical stretching. These measurements highlight how elastic and viscous moduli of MCF-7 breast cancer cells can vary 1,000-fold and 100-fold, respectively. We discuss the sources of these variations, including the level of applied mechanical stress, the rate of deformation, the geometry of the probe, the location probed in the cell, and the extracellular microenvironment.

AB - The mechanical properties of cells influence their cellular and subcellular functions, including cell adhesion, migration, polarization, and differentiation, as well as organelle organization and trafficking inside the cytoplasm. Yet reported values of cell stiffness and viscosity vary substantially, which suggests differences in how the results of different methods are obtained or analyzed by different groups. To address this issue and illustrate the complementarity of certain approaches, here we present, analyze, and critically compare measurements obtained by means of some of the most widely used methods for cell mechanics: atomic force microscopy, magnetic twisting cytometry, particle-tracking microrheology, parallel-plate rheometry, cell monolayer rheology, and optical stretching. These measurements highlight how elastic and viscous moduli of MCF-7 breast cancer cells can vary 1,000-fold and 100-fold, respectively. We discuss the sources of these variations, including the level of applied mechanical stress, the rate of deformation, the geometry of the probe, the location probed in the cell, and the extracellular microenvironment.

U2 - 10.1038/s41592-018-0015-1

DO - 10.1038/s41592-018-0015-1

M3 - Article

VL - 15

SP - 491

EP - 498

JO - Nature Methods

T2 - Nature Methods

JF - Nature Methods

SN - 1548-7091

ER -

Wu PH, Aroush DRB, Asnacios A, Chen WC, Dokukin ME, Doss BL et al. A comparison of methods to assess cell mechanical properties. Nature Methods. 2018 Jun 18;15:491-498. Available from, DOI: 10.1038/s41592-018-0015-1